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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
other: ECHA inquiry result
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Annex V. B10
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
413-060-1
EC Name:
-
Cas Number:
19186-97-1
Molecular formula:
C15H24O4PBr9
IUPAC Name:
tris[3-bromo-2,2-bis(bromomethyl)propyl] phosphate

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella Typhimurium: TA1535, TA1537, TA1538, TA98, TA100
Metabolic activation system:
S-9 from Aroclor 1254 induced rat liver and cofactors
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 50 ... 5000 μg/plate
Concentration range in the main test (without metabolic activation): 50 ... 5000 μg/plate
Vehicle / solvent:
Solvent: DMSO
Details on test system and experimental conditions:
Concentration of the test substance resulting in precipitation: 500 μg/plate

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
>5000 ug/plate
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
>5000 ug/plate
Additional information on results:
Observations:
Heavy precipitate was observed at 5000 ig/plate. No toxicity
was observed at this dose based on reduction in spontaneous
revertants. Slight to moderate precipitate was observed at
500 ig/plate and 1667 ig/plate.
Remarks on result:
other: other: preliminary test
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation