(methoxymethyl)triphenylphosphonium chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(methoxymethyl)triphenylphosphonium chloride

EC Number:

223-664-5

EC Name:

(methoxymethyl)triphenylphosphonium chloride

Cas Number:

4009-98-7

Molecular formula:

C20H20OP.Cl

IUPAC Name:

(methoxymethyl)triphenylphosphanium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: young adult animals (approx. 8 weeks old)
- Weight at study initiation: males mean of 288 g, females mean of 165-183 g
- Fasting period before study: overnight (for a maximum of 20 h)
- Housing:3 animals per sex/cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21°C +/- 3°C
- Humidity (%): 30-70%
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 17.12.2002 To: 07.01.2003

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg b.w.

Doses:

2000 mg/kg (10mL/kg) b.w.
200 mg/kg (10mL/kg) b.w.

No. of animals per sex per dose:

3 females 2000 mg/kg b.w.
3 females 200 mg/kg b.w.
3 males 200 mg/kg b.w.

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations: at 0, 2 and 4 h after dosing and once daily thereafter
- Frequency of weighing: day 1, day 8 and day 15
- Necropsy of survivors performed: yes
- Other examinations performed: gross examination of organs were subjected to necropsy and descriptions of all internal macroscopic
abnormalities recorded

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 200 - ca. 2 000 mg/kg bw

Based on:

test mat.

Mortality:

All animals at 2000 mg/kg b.w. were found dead. The decedents were found dead within 2 hour post treatment. No further mortality occurred at 200 mg/kg b.w..

Clinical signs:

other: 2000 mg/kg b.w.: lethargy, flat and hunched posture, salivation, moribund, slow breathing, piloerection, 200 mg/kg b.w.: restless, hunched posture, chromodacryorrhoea, lethargy The surviving animals had recovered from the symptoms between days 2 and 3.

Gross pathology:

No abnormalities were found at macroscopic post mortem examination of the animals.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

At 2000 mg/kg b.w. all animals died within 2 hours after dosing. At 200 mg/kg b.w. no mortality occurred and therefore the LD50 was considered to be in the range of 300 - 2000 mg/kg b.w..

Executive summary:

At 2000 mg/kg b.w. all animals died within 2 hours after dosing. At 200 mg/kg b.w. no mortality occurred and therefore the LD50 was considered to be in the range of 300 - 2000 mg/kg b.w.. Also at 200 mg/kg b.w. clinical signs such as restlessness, hunched posture, chromodacryorrhoea and lethargy were noted.