Methyl (S)-(-)-lactate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

The organism used was the fresh-water crustacean Daphnia magna, cultured in the laboratory under standard conditions, according to the principles of NPR 6503. The animals were less than 24 h old at the beginning of the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

> 7.5 mg/L

Salinity:

Not applicable

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentrations: No data.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, fill volume: No data
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: No data
- No. of vessels per concentration (replicates): No data
- No. of vessels per control (replicates): No data

TEST MEDIUM/WATER PARAMETERS
- Source/preparation of dilution water: No data

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilization at 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

749 mg/L

95% CI:

>= 592 - <= 788

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Remarks:

The validity criteria could not be checked due to insufficient documentation.

Conclusions:

The 48-h EC50 (immobilization) of methyl-(S)-lactate to Daphnia magna is 749 mg/L.

Executive summary:

The 48-hr acute toxicity of methyl (S)-lactate to Daphnia magna was studied under static conditions. Test organisms were exposed to control and test chemical at unspecified concentrations for 48 hours. Mortality/immobilization and sublethal effects were observed at test termination. The 48-hour EC₅₀ was 749 mg/L. Information on sublethal effects is not available.

Based on the results of this study, methyl (S)-lactate would not be classified as hazardous to invertebrates in accordance with the classification system of the CLP Regulation.

Results synopsis

Test organism age: No data

Test type: Static

EC₅₀: 749 mg/L (95% C.I.: 663–847 mg/L)

Endpoint(s) effected: Immobilisation

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Qualifier:

according to guideline

Guideline:

other: ECHA guidance on information requirements and chemical safety assessment, chapter R.6: QSARs and grouping of chemicals

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

C(=O)(C(C)O)OC

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

811 mg/L

Remarks on result:

other: calculated

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for methyl (S)-lactate was estimated at 811 mg/L, showing very low acute toxicity to invertebrates.

Executive summary:

For determination of the acute toxicity to invertebrates the 'SciQSAR model for acute toxicity in Daphnia magna (48 h immobilisation, EC50)' as included in the Danish QSAR Database has been applied. Based on the calculated 48-h EC50 value of 811 mg/L it can be concluded that methyl-(S)-lactate shows very low acute toxicity to daphnia, not fulfilling the CLP criterion for environmentally hazardous substances of EC₅₀ ≤ 100 mg/L.

Description of key information

The 48-h EC50 value for methyl (S)-lactate for daphnids was predicted to be 811 mg/L. This matches well with an experimental EC50 value of 749 mg/L as reported in the supporting study (Bowmer et al., 1998).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

ca. 811 mg/L