4-amino-2,5-dimethoxy-N-methylbenzenesulphonamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-2,5-dimethoxy-N-methylbenzenesulphonamide

EC Number:

256-435-3

EC Name:

4-amino-2,5-dimethoxy-N-methylbenzenesulphonamide

Cas Number:

49701-24-8

Molecular formula:

C9H14N2O4S

IUPAC Name:

4-amino-2,5-dimethoxy-N-methylbenzene-1-sulfonamide

Details on test material:

purity: 97.8 - 98.0 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

applied concentrations:
0 mg/L
0 mg/L with 0.095 ml Tween 80
100 mg/L nominal
500 mg/L nominal = 186 mg/L measured

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation: born 1989-12-22 and 1989-12-29
- Length at study initiation : 2.5 - 3.5 cm average: 3.0 cm, s=0.32
- Feeding during test: Tetra Min, Tetra Werke, Melle; twice a day, ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.2 - 22.6 °C

pH:

7.4 - 8.0

Dissolved oxygen:

6.6 - 9.1 mg/L

Nominal and measured concentrations:

500 mg/L nominal = 186 mg/L measured

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 10 liter glas container (30 x 22 x 24 cm³)
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according ISO/DIS 7346/1, aerated until oxygen saturATION

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light
- Light intensity: about 700 Lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

186 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Slight change in the behaviour compared with the control groups could be observed

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the result of this test the substance has not to be classified for toxicity to fish.

Executive summary:

The substance was tested for acute toxicity to fish according to OECD guideline 203 over a period of 96 hours. Two nominal concentrations were used : 100 mg/L and 500 mg/L. For the higher one an effective concentration of 186 mg/L was measured. After 96 hours no mortality occured in both test groups. The LC50 is >500 mg/L (nominal) rsp. > 186 mg/L (measured)