Nicotinonitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

no

Remarks:

precede establishment of GLP

Test material

Test material information

Constituent 1

Reference substance name:

Nicotinonitrile

EC Number:

202-863-0

EC Name:

Nicotinonitrile

Cas Number:

100-54-9

Molecular formula:

C6H4N2

IUPAC Name:

pyridine-3-carbonitrile

Constituent 2

Reference substance name:

Pyridine-3-carbonitrile

IUPAC Name:

Pyridine-3-carbonitrile

Test animals / tissue source

Species:

rabbit

Strain:

other: albino

Details on test animals or tissues and environmental conditions:

Body weight: 2.3 to 2.55 kg each, 4-5 months of age.

Test system

Vehicle:

unchanged (no vehicle)

Amount / concentration applied:

0.1 ml or 0.1 g.

Observation period (in vivo):

1, 24, 48 and 72 hours and 21 days

Number of animals or in vitro replicates:

3

Details on study design:

3-Cyanopyridine was tested for eye irritant properties in rabbits. 0.1 g was introduced into the right conjunctival sac of 3 tested animals. Evaluations were made at 1, 24, 48 and 72 hours after application, according to the method of Draize. Evaluation of score is as follows: 1-10 Not irritating; 11-25 Slightly irritating; 26-56 Moderately irritating; 57-110 Severely irritating.

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Score:

65

Max. score:

110

Reversibility:

not reversible

Remarks on result:

other: severe irritation

Any other information on results incl. tables

Cornea: turbidity translucent with easily recognizable on region, district or opalescent opaque cornea, the entire cornea was affected in each area, sprouting of vessels in the cornea

 

Iris: Short term no reaction to light and moderate hyperaemia surrounding the cornea.

 

Conjunctiva: Diffuse, flesh-colored redness, all sections bloody sometimes, significant swelling with partial eversion of the eyelid

 

Other changes in the eye area: Mild serous hypersecretion, loss of hair around the eye

 

The reaction of the cornea after 21 days was not yet completely subsided.

 

The calculated irritation index amounts to 65. 

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

3-Cyanopyridine was tested for eye irritant properties in rabbits after a single exposure. The results indicate strong irritating properties of the rabbit eye.