2-Isobutyryl-3,N-diphenylacrylamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-05-23 to 1995-08-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): PD 123408
- Physical state: White Poweder
- Composition of test material, percentage of components: not given

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: 10% Tween 80 in Dimethylformamide

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauß
- Source: Institute national de Recherche Chimique Appliquee, France
- Age at study initiation (mean and range, SD): < 10 h
- Method of breeding: Fed daily with a suspension of mixed algae (predominantely Scenedesmus and Selenastrum spp.)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

137 mg CaCO3/L

Test temperature:

20 +/- 1 °C

pH:

7.4 - 7.8

Dissolved oxygen:

8.5-9.0 mg O2/l

Nominal and measured concentrations:

Noiminal 1.0, 2.2, 4.6, 10 and 22 mg/l
Measured 0.89, 1.9, 4.0, 7.9 and 16 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass jar 200 ml
- No. of organisms per vessel:20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 20 ml/daphnia


TEST MEDIUM / WATER PARAMETERS: Elendt M7 medium
H3BO3 0.71 mg/l
MnCl4.4H2O 0.09 mg/l
LiCl 0.077 mg/l
RbCl 0.018 mg/l
SrCl2.6H2O 0.038 mg/l
NaBr 0.0040 mg/l
Na2MoO4.2H2O 0.016 mg/l
CuCl2.2H2O 0.0042 mg/l
ZnCl2 0.013 mg/l
CoCl2.6H2O 0.010 mg/l
KI 0.0033 mg/l
Na2SeO3 0.0022 mg/l
NH4VO3 0.00058 mg/l
Fe-EDTA solution 1.7 mg/l


CaCl2.2H2O 294 mg/l
MgSO4.7H2O 123 mg/l
KCl 5.8 mg/l
NaHCO3 64.8 mg/l
NaSiO3.5H2O 6.83 mg/l
NaNo3 0.274 mg/l
KH2PO4 0.143 mg/l
K2HPO4 0.184mg/l

OTHER TEST CONDITIONS
- Adjustment of pH: Hydrogenphosphate buffer
- Photoperiod: 16 h light, 8 h dark



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 (0, 24, 48 h); NOEC (0, 24, 48 h)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

7 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.3 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

16 mg/L

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.9 mg/L

Details on results:

%Concentration loss over test: 13 ... 37

Reported statistics and error estimates:

EC50 values and 95% confidence limits were calculated according to the method of Thompson and Weil.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 (immobilisation) value for PD 132408 with Daphnia magna is 6.3 mg/l. The substance is toxic for invertebrates und the conditions used.

Executive summary:

In a 48 h static acute toxicity tests to daphnia according to OECD guideline 202 with PD 132408 (BIBEA) effects were seen at concentrations of 4.6 mg/l and more. The NOEC is 1.9 mg/l BIBEA (measured) The EC50(48h) was calculated to be 6.3 mg/l. The substance is toxic to invertebrates of species Daphnia magna under the conditions used.