Benzamide, N-[[4-[(cyclopropylamino)carbonyl]phenyl]sulfonyl]-2-methoxy-

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

14 Jul - 27 Oct 2010

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

other: Testing of an addional test species

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.4100 (Terrestrial Plant Toxicity Tier I (seedling emergence))

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on preparation and application of test substrate:

- Method of application to soil surface (if used): The spray solution was applied once; attest initiation on the soil surface.
For the untreated controls for each species a spray solution of 100 L deionized water/ha was applied. The test item was dissolved in deionised water and was applied once with 100 L/ha using a spray chamber (Schachtner Tracksprayer SprayLab SLGH 2500) equipped with an overhead nozzle (Teejet 8001 EVS), with nozzle height set at 35 cm above the sprayed surface. Application speed: 3.0 km/h; Spray pressure: 2.9 bar.

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

21 d

Test temperature:

23 ± 8°C day, 18 ± 8°C night (temperature control set)

pH:

7.24

Details on test conditions:

TEST SYSTEM
- Testing facility: Greenhouse
- Test container: Commercial plastic flower pots (10.5 cm diameter)
- Method of seeding: Seeds were introduced manually in the soil. The seeds were pressed into the soil surface and covered with a layer of the used soil. Soil was top watered immediately to facilitate germination.
- No. of seeds per container: 5 seeds per treatment
- No. of plants (retained after thinning):
- No. of replicates per treatment group: 8
- No. of replicates per control: 8

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Locally supplied soil steam pasteurized before use
- Soil texture (if natural soil)
- % sand: 43.2%
- % silt: 40.2%
- % clay: 16.6%

GROWTH CONDITIONS
- Photoperiod: At least 16 h light (glasshouse control seftings)
- Light source: Natural daylight supplemented by artificial lighting to provide the required photoperiod.
- Light intensity and quality: Regulation: at light intensities >15000 lux the lamps turn off and when >50000 lux the shading closes.
- Day/night temperatures: 23 ± 8°C day, 18 ± 8°C night (temperature control set)
- Relative humidity (%): 70 ± 30% (glasshouse control settings)
- Watering regime and schedules: Watered when required, Pots placed in plastic saucers in which the water was added
- Water source/type: Local tap water.


EFFECT PARAMETERS MEASURED: Emergence, survival, phytotoxicity, growth stages, shoot Iength and shoot dry weight

- Phytotoxicity rating system: Phytotoxicity was assessed according to EPPO Standard 135.
0% (no injury or effect); 10, 20% (indicates slight effect); 30, 40% (indicates moderate effect); 50, 60% (indicates severe effect); 70, 80% (indicates total plant effect); 90% (moribund or dying plant).

Nominal and measured concentrations:

Nominal concentration: 160g a.i./ha
Analysis of the AE 0001789 at the tested application rate revealed to be 100.7 % of nominal.

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

160 g/ha

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

seedling emergence

Remarks:

(no phytotoxicity symptoms)

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

213.3 µg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

seedling emergence

Remarks:

(no phytotoxicity symptoms)

This study revealed no phytotoxicity symptoms as a result of a soil application of 160g a.i./ha AE 0001789 500 SC. Emergence for Alilum cepa was increased by 6.9% in comparison to the untreated control. Survival was increased by 0.4%. Shoot length was reduced by 6.6% which was statistically not significant. Shoot dry weight, with 11.8% reduction was also statistically not significant. There was no effect on growth stage development in comparison to the control.

Table 1: Validity criteria for OECD 208

Criterion	Outcome	Validity criterion fulfilled
Seedling emergence in the controls is ≥ 70%.	Seedling emergence in the controls was 72.5%.	yes
No visible phytotoxic effects (e.g. chlorosis, necrosis, wilting, deformatons) in control seedlings and the plants of every particular species exhibit normal variation in growth and morphology.	No phytotoxic effects (e.g. chlorosis, necrosis, wilting, deformatons) in control seedlings were visible.	yes
In the controls, the mean survival of emerged control seedlings is ≥ 90% for the duration of the study.	In the controls, the mean survival of emerged control seedlings was 93.1% for the duration of the study.	yes
The environmental conditions for a particular species are identical and the growing media contain the same amount of soil matrix, support media or substrate from the same source.	Environmental conditions for all replicates were the same.	yes

 

Validity criteria fulfilled:

yes

Remarks:

See Table 1 in "Any other information on results incl. tables".

Reference 2

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

Analysis of the spray solution

Vehicle:

no

Details on preparation and application of test substrate:

The plants were exposed to diluted spray solutions of AE 0001789 SC 500 g/L (code: TOX 07428-00). The application was conducted with a tracksprayer SprayLab SLGH 2500) equipped with an overhead nozzle (Teejet 8001 EVSV, colour orange) with a water volume of 100 L/ha and a speed of 2.5 km/h. Nozzles were placed 35 cm over the surface. The pressure was 2.45 bar. One rate only (160 g a.s. / ha) was tested for all species. In addition deionised water as a control was tested.

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Species:

Lolium perenne

Plant group:

Monocotyledonae (monocots)

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Species:

Lycopersicon esculentum

Plant group:

Dicotyledonae (dicots)

Species:

Helianthus annuus

Plant group:

Dicotyledonae (dicots)

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Species:

other: Fagopyrum esculentum

Plant group:

Dicotyledonae (dicots)

Species:

Cucumis sativus

Plant group:

Dicotyledonae (dicots)

Species:

Brassica napus

Plant group:

Dicotyledonae (dicots)

Species:

Beta vulgaris

Plant group:

Dicotyledonae (dicots)

Test type:

vegetative vigour test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

21 d

Test temperature:

23 ± 8°C day, and 18 ± 8°C night

pH:

7.31

Details on test conditions:

TEST SYSTEM
- Test container (type, material, size): commercial plastic flower pots with a diameter of 13 cm
- No. of seeds per container: 5 (Beta vulgaris, Brassica napus, Fagopyrum esculentum, Allium cepa, Avena sativa, Lolium perenne, Zea mays), 4 (Cucumis sativus, Glycine max, Helianthus annuus, Lycopersicon esculentum)
- No. of replicates per treatment group: 8 (Beta vulgaris, Brassica napus, Fagopyrum esculentum, Allium cepa, Avena sativa, Lolium perenne, Zea mays), 10 (Cucumis sativus, Glycine max, Helianthus annuus, Lycopersicon esculentum)
- No. of replicates per control: 8 (Beta vulgaris, Brassica napus, Fagopyrum esculentum, Allium cepa, Avena sativa, Lolium perenne, Zea mays), 10 (Cucumis sativus, Glycine max, Helianthus annuus, Lycopersicon esculentum)

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Bayer CropScience GmbH, Global Biology Herbicides, Horticulture; Frankfurt am Main
- Soil texture (if natural soil)
- % sand: 16.7
- % silt: 59.1
- % clay: 24.2
- Soil taxonomic classification: sandy-silt loam
- Organic carbon (%): 1.3

GROWTH CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Day/night temperatures: 23 ± 8°C day, and 18 ± 8°C night
- Relative humidity (%): 29 - 100

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
survival, visual phytotoxicity, growth, shoot biomass and shoot length

Nominal and measured concentrations:

Nominal: 160 g/ha
Measured: Analysis of the spray solution resulted in rates of 99.4 to 100.3% of nominal.

Species:

Beta vulgaris

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Cucumis sativus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

other: Fagopyrum esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lolium perenne

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Zea mays

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: phytotoxicity, survival, shoot biomass (dry weight) and growth stages

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Validity criteria fulfilled:

not specified

Conclusions:

Cyprosulfamide treatment by itself (SC 500g/L) had no significant effects on vegetative vigour following post-emergent application at a maximum rate of 160g cyprosulfamide/ha.

Reference 3

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

Analysis of the spray solution

Vehicle:

no

Details on preparation and application of test substrate:

The seeds were exposed to diluted spray solutions of AE 0001789 SC 500 g/L (code: TOX 07428-00). The application was conducted with a tracksprayer SprayLab SLGH 2500) equipped with an overhead nozzle (Teejet 8001 EVSV, colour orange) with a water volume of 100 L/ha and a speed of 1.5 to 2.5 km/h. Nozzles were placed 35 cm over the surface. The pressure was 2.4 -2.95 bar.

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Species:

Lolium perenne

Plant group:

Monocotyledonae (monocots)

Species:

Hordeum vulgare

Plant group:

Monocotyledonae (monocots)

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Species:

Lycopersicon esculentum

Plant group:

Dicotyledonae (dicots)

Species:

Helianthus annuus

Plant group:

Dicotyledonae (dicots)

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Species:

other: Fagopyrum esculentum

Plant group:

Dicotyledonae (dicots)

Species:

Cucumis sativus

Plant group:

Dicotyledonae (dicots)

Species:

Brassica napus

Plant group:

Dicotyledonae (dicots)

Species:

Beta vulgaris

Plant group:

Dicotyledonae (dicots)

Test type:

seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

21 d

Test temperature:

23 ± 8 °C day, 18 ± 8 °C night

pH:

7.31

Details on test conditions:

TEST SYSTEM
- Testing facility: Bayer CropScience GmbH; Frankfurt am Main, Germany
- Test container (type, material, size): commercial plastic flower pots with a diameter of 10 cm
- Method of seeding: Seeds were sown onto moist soil before covering with a depth of soil equivalent to the diameter of the seed. Prior to spray application pots will be top watered to moisten the upper layer of soil.
- No. of seeds per container: 5
- No. of replicates per treatment group: 8
- No. of replicates per control: 8

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Bayer CropScience GmbH, Global Biology Herbicides, Horticulture; Frankfurt am Main
- Soil texture (if natural soil)
- % sand: 16.7
- % silt: 59.1
- % clay: 24.2
- Soil taxonomic classification: sandy-silt loam
- Organic carbon (%): 1.3
- pH: 7.31

GROWTH CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Day/night temperatures: 23 ± 8 °C day, 18 ± 8 °C night
- Relative humidity (%): 29 - 96

EFFECT PARAMETERS MEASURED:
After application the pots were placed in a green house. Day 0 was defined for each species separately as the day when 70% of the control plants had emerged. The test lasted 21 days. At day 7 and 14 phytotoxicity and survival at all assessment dates and shoot biomass (dry weight) and growth stages at test termination were assessed.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Nominal and measured concentrations:

Nominal application rates:
Sugar beet 1st run, oilseed rape, cucumber, buckwheat 1st run, soybean 1st run, sunflower, tomato, oat, barley, perennial ryegrass, corn: 0 (control), 160 g/ha
Sugar beet 2nd run, buckwheat 2nd run, soybean 2nd run: 0 (control), 10, 20, 40, 80 and 160 g/ha

Measured: Analysis of spray solution at the highest rate showed that rates of 99.8 – 102.7% of nominal were applied.

Species:

other: Fagopyrum esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

other: Fagopyrum esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Cucumis sativus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Cucumis sativus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Beta vulgaris

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Beta vulgaris

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Zea mays

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Zea mays

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Hordeum vulgare

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Hordeum vulgare

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lolium perenne

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Species:

Lolium perenne

Duration:

21 d

Dose descriptor:

ER50

Effect conc.:

> 160 g/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other:

Remarks:

Concentration corresponds to >312.3 µg/kg soil; applied with formulated product

Table 1: Validity criteria for OECD 208

Criterion	Outcome	Validity criterion fulfilled
Seedling emergence in the controls is ≥ 70%.	Seedling emergence in the controls was ≥ 70%.	yes
No visible phytotoxic effects (e.g. chlorosis, necrosis, wilting, deformatons) in control seedlings and the plants of every particular species exhibit normal variation in growth and morphology.	There were no visible phytotoxic effects in control seedlings and the plants of every particular species exhibit normal variation in growth and morphology.	yes
In the controls, the mean survival of emerged control seedlings is ≥ 90% for the duration of the study.	In the controls, the mean survival of emerged control seedlings was ≥ 90% for the duration of the study.	yes
The environmental conditions for a particular species are identical and the growing media contain the same amount of soil matrix, support media or substrate from the same source.	The environmental conditions for a particular species were identical.	yes

 

Validity criteria fulfilled:

yes

Remarks:

See Table 1 in "Any other information on results incl. tables".

Conclusions:

Cyprosulfamide treatment by itself (SC 500g/L) had no significant effects on seedling emergence and growth following pre-emergent application following post-emergent application at a maximum rate of 160g cyprosulfamide/ha.

Description of key information

NOEC of the test substance was determined to be 160 g a.i./ha (213.3 µg/kg soil).

Additional information

The toxicity of Cyprosulfamide to terrestrial plants was assessed in a weight of evidence approach. 

All available studies were conducted with a formulated product containing the registered substance. 

Effects of the test substance on the vegetative vigour (M-283542-01-2) of ten plant species was investigated according to OECD 227. The tested plant species were the four monocots Zea mays, Lolium perenne, Allium cepa, Avena sativa and the seven dicots Lycopersicon esculentum, Helianthus annuus, Glycine max, Fagopyrum esculentum, Cucumis sativus, Brassica napus, Beta vulgaris. The study was conducted as a limit test using natural soil as substrate. Young plants (leaf stage 2 – 4) were sprayed with diluted solutions equivalent to rates of 160 g a.s./ha (213.3 µg/kg soil, using the calculation 1 kg/ha = 1.333 mg/kg soil). In addition deionised water as a control was tested. No adverse effects, exceeding the 25% trigger values for further testing, on survival, visual phytotoxicity, growth, shoot biomass and shoot length were determined 21 after application. The determined ER50 (21 d) was > 160 g a.s./ha.

In a second study (M-283760-01-2) the effects of the test substance on seedling emergence of 11 terrestrial non-target plants were investigated according to OECD 208. Test species were four monocots: Zea mays, Lolium perenne, Hordeum vulgare, Avena sativa and seven dicots Lycopersicon esculentum, Helianthus annuus, Glycine max, Fagopyrum esculentum, Cucumis sativus, Brassica napus, Beta vulgaris. Natural soil containing seeds was sprayed with a diluted solution equivalent to rates 160 g a.s./ha (213.3 µg/kg soil, using the calculation 1 kg/ha = 1.333 mg/kg soil). Effects >25% were only observed in the tier 1 test for Fagopyrum esculentum, Beta vulgaris and Glycine max. A dose-response study (tier 2) was conducted with Fagopyrum esculentum, Beta vulgaris and Glycine max using rates between 10 and 160 g/ha. No significant impacts on survival, emergence, shoot dry weight and shoot length of the three test species was determined in the tier 2 study. Thus, it can be concluded that for all eleven species the ER50 is > 160 g a.s./ha (213.3 µg/kg soil). 

In a repetition of this study, Alilum cepa was tested as a further plant species. The  study (M-397742-01-1) was conducted in accordance to OECD 208 and GLP using natural soil as substrate. The study investigated the emergence and growth of Alilum cepa at a limit concentration of 160 g a.s./ha (213.3 µg/kg soil). The test substance was applied by spray application at test start. Emergence of Alilum cepa was increased by 6.9% in comparison to the untreated control. Survival was increased by 0.4%. Shoot length was reduced by 6.6% which was statistically not significant. Shoot dry weight, with 11.8% reduction was also statistically not significant. There was no effect on growth stage development in comparison to the control. The test substance shows no toxic effects on seedling emergence and growth. 

The available studies demonstrate that the registered substance has no herbicidal activity. On the basis of the data presented, a reliable assessment of toxicity to plants is considered feasible, therefore further testing has been omitted.