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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Link to relevant study record(s)

Description of key information

OECD guideline 201, Pseudokirchneriella subcapitata, static, GLP, RL1, in the presence of 4 mg/L humic acid; read across MDIPA Esterquat C18 unsatd
72 h NOEC (growth rate) = 0.39 mg/mL, 72 h ErC10 = 0.54 mg/L (95% c.i. 0.10-2.9 mg/L), 72 h ErC50 = 1.2 mg/L (95% c.i. 0.24-6.0 mg/L);
72 h NOEC (biomass) = 0.39 mg a.i./mL, 72 h EyC10 = 0.49 mg/L (95% c.i. 0.22-1.0 mg/L), 72 h EyC50 = 0.95 mg/L (95% c.i. 0.44-2.1 mg/L)
OECD guideline 201, Pseudokirchneriella subcapitata, static, GLP, RL1, in the presence of 4 mg/L humic acid; read across MDIPA Esterquat C16-18 and 18 unsatd.:
72 h NOEC (growth rate) = 2.7 mg/mL, 72 h ErC10 = 3.2 (95% c.i. 2.5-4.1 mg/L), 72 h ErC50 = 8.1 mg/L (95% c.i. 6.4-10 mg/L);
72 h NOEC (biomass) = 0.91 mg a.i./mL, 72 h EyC10 = 1.3 (95% c.i. 0.45-3.7 mg/L), 72 h EyC50 = 4.4 mg/L (95% c.i. 1.6-12 mg/L)

Key value for chemical safety assessment

EC50 for freshwater algae:
1.2 mg/L
EC10 or NOEC for freshwater algae:
0.54 mg/L

Additional information

No experimental data are available for the target substance MDEA Esterquat C18 satd.. However, toxicity studies in freshwater algae are available for the structurally similar source substances MDIPA-Esterquat C16-18 and C18 unsatd. and MDIPA-Esterquat C18 unsatd. A justification for read-across is given in the general endpoint summary Aquatic toxicity.

In a 72 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to MDIPA Esterquat C18 unsatd. at nominal concentrations of 0 (control), 0.10, 0.32, 1.0, 3.2 and 10 mg/L, corresponding to measured (TWA) 0.042, 0.14, 0.39, 1.3 and 1.7 mg/L in the presence of 4 mg/L humic acid under static conditions in accordance with OECD guideline 201 (adopted March 23, 2006; Annex 5 corrected 28 July 2011).

Microscopic observations revealed no abnormalities.

The NOEC, EC10 and EC50 based on growth rate were 0.39, 0.54 (95% c.i. 0.10-2.9 mg/L) and 1.2 mg/L (95% c.i. 0.24-6.0 mg/L), respectively.

The NOEC, EC10 and EC50 based on based on yield were 0.39, 0.49 (95% c.i. 0.22-1.0 mg/L) and 0.95 mg/L (95% c.i. 0.44-2.1 mg/L), respectively.

All effect levels are given based on measured (TWA) concentrations.

In a 72 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to MDIPA-Esterquat C16-18 and C18 unsatd. at nominal concentrations of 0 (control), 0.25, 0.80, 2.5, 8.0 and 25 mg/L, corresponding to measured (TWA) 0.29, 0.91, 2.7, 6.2 and 25 mg/L in the presence of 4 mg/L humic (<2 mg/L DOC) acidunder static conditions in accordance with OECD guideline 201 (adopted March 23, 2006; Annex 5 corrected 28 July 2011).

Microscopic observations revealed no abnormalities.

The NOEC, EC10 and EC50 based on growth rate were 2.7, 3.2 (95% c.i. 2.5-4.1 mg/L) and 8.1 mg/L (95% c.i. 6.4-10 mg/L), respectively.

The NOEC, EC10 and EC50 based on based on yield were 0.91, 1.3 (95% c.i. 0.45-3.7 mg/L) and 4.4 mg/L (95% c.i. 1.6-12 mg/L), respectively.

All effect levels are given based on measured (TWA) concentrations.