Registration Dossier
Registration Dossier
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EC number: 222-581-1 | CAS number: 3539-43-3
Endpoint summary
Administrative data
Description of key information
Daphnia: The substance showed no adverse effects on the test organisms at over the limit of reported water solubility when tested by WAF method.
Actual values from the test report suggest that the effect concentration was higher than 100 mg/l. However, no test method was used to determine the level of substance in solution, and it is known that the actual solublity of this substance is low, of the order of ~100 mg/l. Hence it is highly unlikely that the higher nominal levels of solubilised substance in the dilution water were accurate. There may have been some undissolved substance in the system. In any event, no mortality of daphnia pulex was observed at a reported dosing of 1200 mg/l
Algae: The definitive test was performed according to the OECD 201 (2011) guideline.
The nominal concentration of the test material (100 mg/L) and mean measured concentration of Potassium have been used in the determination of the EC50 and NOEC values
The algal cells were examined microscopically during the determination of cell concentration. All cells within the control and test concentrations appeared normal, no abnormalities were observed
All validity criteria for the definitive test were met. A value of >100 mg/l nominal has been assessed for both EC50 and NOEC values
Additional information
The substance that has been tested for these end points is the potassium salt of the hexadecyl hydrogen phosphate. Once in solution, the potassium ion will dissociate from the substance leaving the hexadecyl hydrogen phosphate anion as the organic species of relevance for the effect on aquatic species, which is basically the substance being registered.
The potassium ion in solution is ubiquitous in aquatic systems and known not to be toxic at low levels. The pH of the mono constituent substance being registered, which is >= 80% hexadecyl hydrogen phosphate, is reported to be around 5.0, slightly lower than the K salt in solution. Both the K salt and the acid substance are of very low solubility. The aquatic test for alga recognised this issue and testing was carried out as per insoluble substances.
The similarity in substances and properties have been assessed and read-across is considered valid.
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