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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1983

Materials and methods

Principles of method if other than guideline:
The experimental techniques followed Standard Methods (APHA - American Public Health Association et al., 1981) and those used for the static renewal 96-h LC50 rainbow trout study except for the following modifications.
GLP compliance:
not specified

Test material

1
Chemical structure
Reference substance name:
2-amino-4,6-dinitrophenol
EC Number:
202-544-6
EC Name:
2-amino-4,6-dinitrophenol
Cas Number:
96-91-3
Molecular formula:
C6H5N3O5
IUPAC Name:
2-amino-4,6-dinitrophenol
Specific details on test material used for the study:
Picramic acid (lot number 38C-0117) was purchased from the Sigma Chemical Company (St. Louis, Missouri),

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
- Source: Wytheville National Fish Hatchery, Max Meadows, Virginia.
- Age at study initiation (mean and range, SD): Juvenile

Study design

Test type:
semi-static
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
177.0 (+ 11.9) mg/L CaCO3
Test temperature:
10.8 (+0.52)°C
pH:
7.9 (+ 0.04)

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
46.2 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
mortality (fish)
Details on results:
Picramic Acid had an increasing trend of toxicity with time.
The Picramic Acid LC, values were 90.9 mg/L (at 24 h), 68.6 mg/L (at 48 h), 56.6 mg/L (at 72 h), and 46.2 mg/L (at 96 h).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
96 hour LC50 of the test item was 46.2 mg/l for Picramic acid (Oncorhynchus mykiss) .
Executive summary:

The short term toxicity test to fish was perfomed with picramic aicd. 96 hour LC50 of the test item was  46.2 mg/l for (Oncorhynchus mykiss) .