N,N'-(2-chloro-1,4-phenylene)bis(3-oxobutaneamide)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000-01-18 to 2000-03-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

443-010-4

EC Name:

-

Cas Number:

53641-10-4

Molecular formula:

C14H15ClN2O4

IUPAC Name:

N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Aquaeous solutions
Volume: 20 mL
Nominal test substance concentrations: 0 mg/L; 22 mg/L; 50 mg/L;100 mg/L and
220 mg/L

Test solutions

Vehicle:

yes

Remarks:

Water

Details on test solutions:

A solubilizer was not used. The test substance was weighed,
water for dilution was added and was homogenized using an Ultra-Turrax.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species
Daphnia magna - Clone 5 (IRChA, France)

Origin
Stock culture of Aventis Pharma Deutschland GmbH, ProTox

Breeding conditions
100 mL beakers (height 90 mm, diameter 50 mm), filled with 70 mL test water (ac-cording to Elendt, M4) served as culture vessels. One daphnid was used per culture vessel. The animals were used for breeding up to an age of 42 days. The reproduction rate and the state of health of the animals were monitored daily apart from weekends. The animals were fed three times a week with monocellular green algae (Scenedes-mus subspicatus).
The supplied diet was based on the amount of organic carbon (C). The ration level is 0.2 mg carbon / day / animal.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.3 mmol Ca2+ + Mg2+ / L

Test temperature:

20.2 - 20.6

pH:

7.7 - 8.0

Dissolved oxygen:

8.5 - 8.8

Nominal and measured concentrations:

Nominal: 22, 50, 100, 200 mg/L
Measured: 22.0, 54.3, 113.5, 223 mg/L

Details on test conditions:

The study was conducted in a static system. As test chambers 250 mL beakers were used (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 mL test water. The room temperature was about 21°C. The room was illuminated by fluorescent tubes for 16 hours each day.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

69.9 mg/L

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

50.9 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

22 mg/L

Reported statistics and error estimates:

Calculation of the effect concentration (EC50) after 24 and 48 hours was carried out by probit analysis (after the method according to LINDER und WEBER, confidence limit according to FIELLER). Due to the steep dose-response curve it was not possible to calculate confidence intervals for the 48 hours EC50.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test the EC50 of CL-DAEP after 48 hours was 50.9 mg/L.

Executive summary:

CL-DAEP was tested in Daphnia magna (water flea) for 48 hours in a static system. The concentrations tested were 22, 50, 100, 220 mg/L and a negative control (0 mg/L).
Before insertion of the test organisms the chamber contents were stirred for approxi-mately 24 hours with a magnetic-stirrer to ensure that the solubility limit of the test substance in the test water was reached.
All tested concentrations were visually present as clear solutions. No particulate matter was observed.
The substance concentration was determined in all tested groups. The values deter-mined were in a range of + 20 % of the theoretical value.
In this 48-hour acute toxicity study of CL-DAEP the following immobility values were determined:

24h:

EC0 = 22.0 mg/L

EC50 = 69.9 mg/L (CI: 51.6 - 94.5 mg/L)

EC100 = > 100.0 mg/L

 

48h:

EC0 = 22.0 mg/L

EC50 = 50.9 mg/L

EC100 = 100.0 mg/L

In the control group no immobility was observed. Immobility was observed at 50, 100 and 220 mg/L. The 22 mg/L group showed no effect in comparison to the negative control.