Cyclododeca-1,5,9-triene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical verification; a solubilizer was used in this study; However, as the effect values were still above water solubility, the results can be regarded worst case and no effects up to the water solubility at 0.28 mg/L

Data source

Reference

Reference Type:

other: secondary source

Title:

Unpublished Data, Sample Designation S6-1248; S. A. No. 68862.

Author:

Cities Service Research and Development Co.

Year:

1961

Bibliographic source:

cited in Robust summary for 1,5,9-cyclododecatriene (revised). U.S. EPA, 46 pp (2003-10-27)

Materials and methods

Principles of method if other than guideline:

Method: no data

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Cyclododeca-1,5,9-triene

EC Number:

225-533-8

EC Name:

Cyclododeca-1,5,9-triene

Cas Number:

4904-61-4

Molecular formula:

C12 H18

IUPAC Name:

cyclododeca-1,5,9-triene

Details on test material:

IUCLID4 Test substance: other TS: 1,5,9-cyclododecatriene, purity 100 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Vehicle, solvent: acetone, 2.5 ml/l

Test organisms

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

- fish were not fed during the test

Study design

Test type:

static

Water media type:

brackish water

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 +/- 1 degree C

Dissolved oxygen:

> 60% saturation for first 48 hours; > 40% saturation  thereafter

Nominal and measured concentrations:

- Nominal concentrations: 0.3125; 0.625; 1.25; 2.5; 5.0 mg/l

- Additionally: 10; 50 mg/l test substance; 5.0; 25 ml/l vehicle control

Details on test conditions:

TEST SYSTEM
- Number of replicates, fish per replicate: 2 replicates with 10 fish each

TEST CONDITIONS
- Intensity of irradiation: daylight 8-100 fc
- Photoperiod: approximately 16 hours daylight / 8 h darkness

TEST PARAMETER
- observations for biological response at 24-h intervals

STATISTICAL ANALYSIS
- LC50 values were determinded using probit analysis

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.02 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1.69-2.42 mg/L

Details on results:

All deaths occurred within 24 hours.

Any other information on results incl. tables

Sublethal observations / clinical signs:

- Effect data (Mortality):
  control, solvent control, 0.3125 mg/l, 0.625 mg/l: 0/20
  1.25 mg/l:  2/20
  2.5  mg/l: 14/20
  5.0  mg/l: 20/20
  
  Additional testing:
  10.0 mg/l: all organisms dead within first hour
  5.0 ml/l solvent control: 10 % mortality (between 24 and 48 hours)
  30.0 mg/l: all organisms dead within 30 minutes
  25 ml/l solvent control: all organisms dead within 40 min
- Other effects: 
  2.5 mg/l: Erratic swimming
  5.0 mg/l: Twitching

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 was determined as 2.02 mg/L indicating that the test substance may be toxic to fish.