Suberic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-03-30 - 2018-04-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Suberic acid

EC Number:

208-010-9

EC Name:

Suberic acid

Cas Number:

505-48-6

Molecular formula:

C8H14O4

IUPAC Name:

octanedioic acid

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Molecular formula:
C8H14O4
Molecular Mass:
174.20 g/mol
Characteristics (Physical Appearance):
White crystalline powder
CAS No.:
505-48-6
Batch Number:
308
Purity:99.90%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

50 mg of SUBERIC ACID was dissolved in acetone to prepare a stock solution of a volume of 5 ml, with a nominal concentration of 10 mg/ml. This stock solution was added in test containers to attain the concentration of 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test Species and Justification for selection of species:
Daphnia magna; One of the Daphnia species recommended by OECD Guideline No. 202.
The daphnids were derived from a healthy stock with very less incidence of mortality, absence of males and discoloured organisms in the culture.
Source:
Bred at INTOX PVT. LTD.
Age at beginning of the test:
Less than 24 hours
No. of Daphnids per Dose Group:
20 (5 Daphnids per replicate)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

234.06 mg/L

Test temperature:

21.4 to 21.7 ºC

pH:

7.20 to 8.12

Dissolved oxygen:

7.67 - 7.98 mg/l

Nominal and measured concentrations:

Day 1: Nominal: 100 mg/l
Measured: 96.79 mg/l
Day 3: Nominal: 100 mg/l
Measured: 96.85 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The percentage of daphnids those were immobilized in the reference item group at 24 hours were 20, 45 and 85 and at 48 hours were 20, 60 and 100 at the concentrations of 0.40, 0.63 and 1.00 mg/L respectively.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Acute Immobilisation Test of SUBERIC ACID in Daphnia magna was performed in compliance with the Organization for Economic Co-operation and Development (OECD) Guideline for testing of chemicals, Section 2, No. 202, 'Daphnia Sp., Acute Immobilisation Test' adopted by the council on 13 April 2004.
The acute immobilisation EC50 value of SUBERIC ACID in Daphnia magna at 24 and 48 hours exposure period is estimated to be greater than 100 mg/L.

Executive summary:

Acute Immobilisation Test of SUBERIC ACID in Daphnia magna was performed in compliance with the Organization for Economic Co-operation and Development (OECD) Guideline for testing of chemicals, Section 2, No. 202, 'Daphnia Sp., Acute Immobilisation Test' adopted by the council on 13 April 2004.

Test item was dissolved in acetone and was tested for acute toxic effects on the swimming capabilities of Daphnia magna employing a static test system. In the main study, four replicates per treatment group with 5 daphnids per replicate were exposed to the SUBERIC ACID at concentration of 100 mg/L. Observations were made for immobilisation at 24 and 48 hours of treatment. A concurrent blank and vehicle controls were also run simultaneously. Potassium dichromate was used as a reference item and tested at the concentrations of 0.40, 0.63 and 1.00 mg/L.

No immobilisation was observed within the 24 and 48 hours at the concentration of 100 mg/L. No immobilisation was observed in blank and vehicle control groups.

The percentage of daphnids those were immobilized in the reference item group at 24 hours were 20, 45 and 85 and at 48 hours were 20, 60 and 100 at the concentrations of 0.40, 0.63 and 1.00 mg/L respectively.

The acute immobilisation EC50 value of SUBERIC ACID in Daphnia magna at 24 and 48 hours exposure period is estimated to be greater than 100 mg/L.