Reaction mass of 3,7-dimethyl-1-octyl acetate and citronellyl acetate

Short-term toxicity to fish

Description of key information

The short-term toxicity to fish is assessed based on read-across from the close structural analogue Citronellyl Acetate (CAS# 150 -84 -5) to Citronellyl Acetate Multi.

Acute fish toxicity of Citronellyl Acetate mono

For Citronellyl Acetate mono, the acute toxicity to fish was examined in a study according to OECD TG 203, and in compliance with GLP criteria. Groups of zebrafish (1 replicate of 7) were exposed to nominal concentrations of 0 (control), 1, 2, 4, 8, and 16 mg/L for 96 hours under semi-static conditions (24-h renewal of test media). Test substance analysis was performed at start and end of the first and the last renewal period for all concentrations except 16 mg/L which was analyzed only over the first period. Analytically determined concentrations of the test substance in the test water were in a range of ± 20% of the nominal concentration at the start of the intervals and decreased to 21.4 - 62.0 % of the nominal concentration at the end of exposure periods. This was considered most likely due to evaporation of the test substance. Arithmetic mean measured concentrations were determined to be 0.68, 1.4, 2.29, 4.61, and 8.14 mg/L for the different nominal concentrations, respectively. Effect values are expressed as measured. Mortality and symptoms of intoxication were recorded at 1, 4, 24, 48, 72 and 96 hours after the start of the test.

Mortality was recorded only at the highest tested concentration of 16 mg/L at which all fish were dead after 48 hours exposure. At this concentration tumbling was recorded observed until fish died. At the test concentration of 8 mg/L tumbling was also recorded but only until 4 hours after the start of the test. Based on these findings, the 96-h LC50 was determined to be 6.1 mg/L based on measured concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

6.1 mg/L