Hydrazinecarboximidamide, 2-[(2-hydroxyphenyl)methylene]-, reaction products with 2-undecanone

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 March 2018-17 April 2018

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Analytical method:

liquid chromatography

mass spectrometry

Key result

Type:

log Pow

Partition coefficient:

< -0.64

Temp.:

20 °C

Remarks on result:

other: pH not reported

Key result

Type:

log Pow

Partition coefficient:

> 4.9

Temp.:

20 °C

Remarks on result:

other: pH not reported

Details on results:

At 20C, log Pow values were estimated by the HPLC estimation method to range from unretained (log Pow <-0.64) to > 4.9 for hydrazine carboximidamide, 2 [(2 hydroxyphenyl)methylene] – reaction products with 2 undecanone.

Conclusions:

The n-octanol/water partition coefficient (Pow) of the test substance was determined by comparison to compounds with known log Pow values based upon retention times on an HPLC analytical column at 25C. The test substance was designated hydrazine carboximidamide, 2 [(2 hydroxyphenyl) methylene] – reaction products with 2-undecanone.
The test substance was dissolved in 75:25 methanol:water. The samples were injected onto a C 18 HPLC column with 70:30 methanol:water as mobile phase. This method resulted in the retention of reference standards with known log Pow values of 0.3 to 4.9 to be retained on the column from approximately 3.5 to 35 minutes. Thiourea (an unretained organic compound) was injected to establish dead time. The test substance was injected onto the column, and the eluent was monitored using a mass spectrometer. Multiple peaks were detected between approximately 3.1 and 45.2 minutes. The first resolved sample peak, observed at approximately 3.1 minutes, eluted before or with the thiourea and was therefore determined to be unretained. The last peak from the sample injection, at approximately 45 minutes, eluted after the reference standard with the highest known log Pow value (4.9). At 20C, the average log Pow value was estimated by the HPLC estimation method to range from unretained to > 4.9 for hydrazine carboximidamide, 2 [(2 hydroxyphenyl)methylene] – reaction products with 2 undecanone.

Executive summary:

At 20C,  log Pow values were estimated by the HPLC estimation method to range from unretained (log Pow <-0.64)  to > 4.9 for hydrazine carboximidamide, 2 [(2 hydroxyphenyl)methylene] – reaction products with 2 undecanone.  

Description of key information

At 20C,  log Pow values were estimated by the HPLC estimation method to range from unretained (log Pow <-0.64)  to > 4.9 for hydrazine carboximidamide, 2 [(2 hydroxyphenyl)methylene] – reaction products with 2 undecanone.