(2,4,6-trioxo-1,3,5-triazine-1,3,5(2H,4H,6H)-triyl)triethane-2,1-diyl tris(3-mercaptopropionate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isooctyl mercaptoacetate

EC Number:

246-613-9

EC Name:

Isooctyl mercaptoacetate

Cas Number:

25103-09-7

Molecular formula:

C10H20O2S

IUPAC Name:

6-methylheptyl sulfanylacetate

Details on test material:

Identification: Isooctyl thioglycolate (IOTG)
CAS no. 25103-09-7
Source: Elf Aquitaine production
Batch: TA 2511
Purity: 99.05 % (w/w)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The test item was applied to the skin of one group of ten Sprague-Dawley rats (five males and five females). The application was performed with the undiluted test item at the dose of 2000 mg/kg, taking into consideration that its specific gravity was 0.96 g/mL. The test site was then covered by a semi-occlusive dressing for 24 hours.

Duration of exposure:

24 h

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Clinical signs, mortality and body weight gain were checked for a period of 14 days following the single application of the test item. All animals were subjected to necropsy.

Statistics:

Not appropriate

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: No death and clinical signs

Mortality:

No deaths occurred at 2000 mg/kg. 

Clinical signs:

other: The general behaviour and body weight gain of the animals were not affected by the treatment. No skin irritation was observed.

Gross pathology:

A macroscopic axamination revealed no abnormalities in the animals sacrificed at the end of the study.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Executive summary:

In an OECD 402 guideline study, undiluted IOTG was applied to the skin of ten Sprague-Dawley male and female rats at a dose of 2000 mg/kg.The test site was then covered by a semi-occlusive dressing for 24 hours. Clinical signs, mortality and body weight gain were checked for a period of 14 days and all animals were subjected to necropsy. No death occurred at 2000 mg/kg. No skin reactions were observed. Clinical signs and body weight gain were not affected by treatment. The LD0 for IOTG was greater than 2000 mg/kg bw.