Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 230-636-6 | CAS number: 7235-40-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1995-05-03 to 1995-11-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline-conform study performed under GLP without deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- β,β-carotene
- EC Number:
- 230-636-6
- EC Name:
- β,β-carotene
- Cas Number:
- 7235-40-7
- Molecular formula:
- C40H56
- IUPAC Name:
- β,β-carotene
- Details on test material:
- - Name of test material (as cited in study report): β-Carotin
- Physical state: brown solid
- Analytical purity: 99.8%
- Purity test date: 040860
- Lot/batch No.: 405108
- Expiration date of the lot/batch: 27/10/95
- Storage condition of test material: room temperature, in darkness
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 18, 32, 58 and 100 mg/L (nominal)
- Sampling method: Composite samples (about 150 mL per concentration and control) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the test vessels. Samples of test solutions were taken immediately before exposure and after 72 hours exposure and stock solution (almost equal to highest test concentration) was taken 24 hours before exposure.
- Sample storage conditions before analysis: All samples were kept at -18 °C to -22 °C until analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200.9 mg test substance and 208 mg of dimethylformamid/Tween 80 (96/4 % w/w) were mixed. This blend was mixed and made up to 200 mL with reconstituted water. Calculated amounts of this stock solution and vehicle to achieve identical vehicle concentrations at all test concentrations were given into the water and were homogeneously distributed. The test solutions were equilibrated for 24 hours under test conditions.
- Differential loading: No
- Controls: vehicle control, water control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethylformamid/Tween 80 (96/4 % w/w)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 96 mg dimethylformamid/L and 4 mg Tween 80/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Some deposit of the test substance was observed at test concentrations 10-100 mg/L at the start and at test concentrations 58 and 100 mg/L at the end of exposure. Parts of the test substance were swimming on the surface of the test water at test concentrations 58 and 100 mg/L at the start and at test concentrations 10-100 mg/L at the end of exposure.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 61.81 SAG / ATCC 22662
- Source (laboratory, culture collection): collection of algae cultures, Pflanzenphysiologisches Institut, Göttingen, Germany
- Method of cultivation: according to guideline
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 35 mg CaO/L
- Test temperature:
- 22 ± 1 °C
- pH:
- Test start: 8.1
Test end: 8.1 - 8.7 - Nominal and measured concentrations:
- Nominal: 10, 18, 32, 58, 100 mg/L
Measured, start of test: 16.6, 37.1, 60.0, 104, 163 mg/L
Measured, end of test: 5.0, 9.1, 13.1, 29.7, 47.8 mg/L
Calculated actual mean concentrations: 10.8, 23.1, 36.6, 66.9, 105.4 mg - Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass 100 mL Erlenmeyer flasks (total volume 125 mL)
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 10600 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Composition according to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: cold white fluorescent light at 120 µE/m²sec (approx. 8000 lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: "TOA" cell counter
TEST CONCENTRATIONS
- Range finding study: no
- Test concentrations: Nominal test concentrations of 10, 18,32, 58 and 100 mg test substance per litre. The measured test substance concentrations were 16.6, 37.1, 60.0, 104, 163 and 5.0, 9.1, 13.1, 29.7 and 47.8 mg of test substance per litre at the start and end of exposure, respectively.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 231 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 180 - 371 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 81 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 63 - 112 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Adherence to test vessels: Some deposit of the test substance was observed at test concentrations 10-100 mg/L at the start and at test concentrations 58 and 100 mg/L at the end of exposure.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Parts of the test substance were
swimming on the surface of the test water at test concentrations 58 and 100 mg/L at the start and at test concentrations 10-100 mg/L at the end of exposure.
Any other information on results incl. tables
The calculated actual mean concentrations 10.8, 23.1, 36.6, 66.9 and 105.4 mg test substance/L were practically within 100 · 20 % of the nominal concentrations, therefore EC50 values were based on nominal concentrations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 (0-72 h) was nominal 231 mg/L and EbC50 (0-72 h) was nominal 81 mg/L.
- Executive summary:
The test was conducted to determine the concentration at which the growth of green algae (Selenastrum capricornutum) is reduced by 50% in relation to control. The test was conducted according to OECD TG 201.
After a preculture period of 3 days, Selenastrum capricornutum (initial cell density 10600 cells/mL) was exposed to nominal test concentrations of 10, 18,32, 58 and 100 mg/L. The measured test substance concentrations were 16.6, 37.1, 60.0, 104, 163 and 5.0, 9.1, 13.1, 29.7, 47.8 mg/L at the start and the end of exposure, respectively. The calculated actual mean concentrations 10.8, 23.1, 36.6, 66.9 and 105.4 mg/L were practically within 100 · 20 % of the nominal concentrations, therefore following values were based on nominal concentrations. The ErC50 (0-72 h) was nominal 231 mg/L and EbC50 (0-72 h) was nominal 81 mg/L. The NOEC was < 10 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.