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EC number: 947-548-5
CAS number: -
Skin irritation - Mean value of three test data gave a value of 78.3%
mean issue viability in the EpiDerm skin test (OECD 439).
Accoridng to the test protocol, a value of >50% predicts Non-Irritant
under GHS classification.
Human patch test data also indicated non-irritant at 10% in water over
Eye irritation - Under the conditions of the Matek EpiOcular test, the
results indicate that the Colaliquid DC-5 100% Active test article, at
100%, has a non-irritating/minimally irritating classification.
Non-Irritant under GHS classification.
The HET-CAM hen's egg test also confirmed non-irritant.
A Molecular Devices SpectraMax M5 Microplate Reader was used to
determine the absorbance of each extract at 570nm. With the absorbance
of the negative control (distilled water) defined as 100%, the percent
absorbencies of the test article were determined. The
percentages listed below directly correlate with the cell metabolism in
the EpiOcular samples.
(20% - 4 hrs.) - % viability = 39, % inhibition = 61
(20% - 1 hr.) - % viability = 61, % inhibition = 39
(20% - 20 mins.) - % viability = 75, % inhibition = 25
When possible, using a semilog scalg the percent viabilities for the
article were plotted on the linear y axis versus the dosing time on the
log x axis. By interpolation, the time at which the percent viability
would be 50% was determined (ET-50).
As a general guideline (provided by MatTek) the following equation can
be used to estimate the rabbit Draize eye score:
Draize : 4.7 4 + 101.7/(ET-50)^0.5
Based on the literature (Kay, J.H. and Calan&a J.C., "lnterpretation of
eye irritation tests,"J Soc. Cosmetic Chem., 13, 281-289 (1962)), the
ocular initancy estimated potential has been categorized by MatTek into
the following groups, based on the Draize score:
Under the conditions of this test, the Colaliquid DC-5 100% Active test
article, at 20%, elicited in vitro results which indicate that its ET-50
is 122.1 minutes. Therefore, at 100%, the test article's estimated
Draize ocular iritation score is approximately 4.5 with a "minimally
irritating" irritancy classification.
Previous studies have shown that the CAM of the hen's egg is more
sensitive to lquid irritants than is the rabbit eye. Therefore, 50%
dilutions of the liquid test and reference articles were used.
Historical in-vivo testing on Johnson's reference product has shown this
material to be 'moderatley irritating' eliciting scores approaching 10
at 24 hours when dosed at 100% and tested using the draize ocular
The Prell reference product under the same in-vivo Draize testing has
given scores approaching 30 at 24 hours when dosed at 100%.
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