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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
4 February 1992 to 3 December 1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
92.7 mg/L
Nominal / measured:
nominal
Conc. based on:
other: enyzme concentrate dry matter
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
31.2 mg/L
Nominal / measured:
nominal
Conc. based on:
other: enzyme concentrate dry matter
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
Analysis of the immobility of Daphnia magna exposed to peroxidase to concentrations up to 546.6 mg enzyme concentrate dry matter/L for 48 hours gave an EC50 value of 92.7 mg enzyme concentrate dry matter/L, equivalent to 26.2 mg/L active enzyme protein.
Executive summary:

The acute toxicity of Peroxidase to Daphnia magna was assessed under static exposure conditions.


The study was conducted in accordance with OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC. A group of twenty Daphnia ≤24 hours old was exposed for 48 hours to Peroxidase in concentrations up to 546.6 mg enzyme concentrate dry matter/L. Dechlorinated tap water was used as test water, having a total hardness of 150 -200 mg/L.


Observations of the Daphnia in each control and test vessel were made after 24 and 48 hours, which produced these findings:


48-hour EC50 value: 92.7 mg/L (26.2 mg/L active enzyme protein)


48-hour NOEC value: 31.2 mg/L (8.8 mg/L active enzyme protein)

Description of key information

The short-term toxicity to aquatic invertebrates of the present target substance, Glucose oxidase IUBMB 1.1.3.4, has not been determined but another enzyme (Peroxidase, IUBMB 1.11.1.7) has been analyzed and used as a source substance for read-across. Analysis of the immobility of Daphnia magna exposed to peroxidase to concentrations up to 546.6 mg enzyme concentrate dry matter/L for 48 hours gave an EC50 value of 92.7 mg enzyme concentrate dry matter/L, equivalent to 26.2 mg/L active enzyme protein.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
92.7 mg/L

Additional information