Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 284-628-2 | CAS number: 84961-40-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1989
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Amines, C10-14-branched and linear alkyl, bis[2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)]chromate(1-) (1:1)
- EC Number:
- 284-628-2
- EC Name:
- Amines, C10-14-branched and linear alkyl, bis[2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)]chromate(1-) (1:1)
- Cas Number:
- 84961-40-0
- Molecular formula:
- C32H22CrN10O8.C10-14H21-29NH2
- IUPAC Name:
- Amines, C10-14-branched and linear alkyl, bis[2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)]chromate(1-) (1:1)
- Test material form:
- solid
- Details on test material:
- - State of aggregation: solid (powdeQ
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No. of test material: Abl. 13-1341
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At the start of the test (after 0 hours) samples from vessels which were not stocked with daphnids were analyzed and at the end of the test (after 48 h) samples from stocked and unstocked vessels were analyzed.
- Sample storage conditions before analysis: refrigerator
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For the preparation of the stock solution (100 mg/L) the test substance was stirred for about 20 h at 22 ± 1 °C in a M4-Medium. Undissolved test substance was removed by paper filtration and centrifugation for about 20 min at about 6000 rpm. By dilution of this stock solution with test medium the following nominal concentrations were prepared: 100 mg/L, 50 mg/L, 25 mg/L, 12.5 mg/L, 6.25 mg/L, 3.13 mg/L, 1.56 mg/L, 0.78 mg/L
- Medium: The synthetic medium M4 was used for the culture and test. The medium was prepared on the basis of an ultrapure, deionized water (conductivity < 0.05 µS/cm). The medium has the composition according to the guideline. The medium was aerated until saturated with oxygen, and was left to stand tor 24 hours for stabilization.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUSS
- Source: Institut National de Recherche Chimique Appliquées, France.
- Culture conditions: The daphnids were cultured under standard conditions in the laboratory.
- Feeding during the test: None
- Age at study initiation: 2 – 24 hours
- Age of the stock of animals: 2 – 4 weeks
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.20 – 3.20 mmol/L
- Test temperature:
- 19.5 – 21.1 °C
- pH:
- 7.85 - 8.5
- Dissolved oxygen:
- 7.3 – 8.3 mg/L
- Salinity:
- - Alkalinity up to pH 4.3: 0.80 – 1.00 mmol/L
- Molar ratio Ca:Mg: ~ 4:1 - Conductivity:
- 550 - 650 µS/cm
- Nominal and measured concentrations:
- - Nominal concentrations: 100, 50, 25, 12.5, 6.25, 3.13, 1.56 and 0.78 mg/L.
- Measured concentrations: The following nominal concentrations of the test substance were analyzed: 100, 12.5, 1.56 and 0 mg/L (control). The analytical recovery rate was after 48 hours in all analyzed test solutions below the detection limit (< 1 %). The effective concentrations mentioned are based therefore on the nominal concentrations of the test substance. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- Test volume: 10 mL
- Aeration: yes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: For the preparation of the stock solution (100 mg/L) the test substance was stirred for about 20 h at 22 ± 1 °C in M4-Medium. Undissolved test substance was removed by paper filtration and centrifugation for about 20 min at about 6000 rpm.
- Alkalinity up to pH 4.3: 0.80 – 1.00 mmol/L
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
- Intervals of water quality measurement:
Measurement of temperature: After 0, 24, 48 hours in an extra vessel close to the test vessels.
Measurement of pH und oxygen: After 0 and 48 hours in one replicate at each concentration.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Illumination/Photoperiod: Artificial light, OSRAM L58 W31 warm white, day:night – rhythm 16:8 hours
- Light intensity: About 5 - 6 µE/(m2-s) in the range of 400 – 700 nm
EFFECT PARAMETERS MEASURED: This study investigated the influence of the test substance on the swimming ability (mobility) of Daphnia magna after 48 hours of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations:
Nominal concentrations: 100, 50, 25, 12.5, 6.25, 3.13, 1.56 and 0.78 mg/L.
Measured concentrations: The following nominal concentrations of the test substance were analyzed: 100, 12.5, 1.56 and 0 mg/L (control). The analytical recovery rate was after 48 hours in all analyzed test solutions below the detection limit (< 1 %). The effective concentrations mentioned are based therefore on the nominal concentrations of the test substance. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% C.L not determined; no toxic effects within the range of solubility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% C.L not determined; no toxic effects within the range of solubility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% C.L not determined; no toxic effects within the range of solubility
- Results with reference substance (positive control):
- not determined
- Reported statistics and error estimates:
- No reported statistics and error were estimated.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is with high probability not acutely harmful to aquatic invertebrates. No toxic effects occurred within the range of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.