Reaction mass of 5,12-dihydroquino[2,3-b]acridine-7,14-dione and aluminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2-sulphonate) and dialuminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2,9-disulphonate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 Mar 2017 to 24 Mar 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 5,12-dihydroquino[2,3-b]acridine-7,14-dione and aluminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2-sulphonate) and dialuminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2,9-disulphonate)

EC Number:

909-092-5

Molecular formula:

Unspecified

IUPAC Name:

Reaction mass of 5,12-dihydroquino[2,3-b]acridine-7,14-dione and aluminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2-sulphonate) and dialuminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2,9-disulphonate)

Test material form:

solid

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
The sample of the test item was stored at ambient temperature without exposure to light in a tightly sealed container.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The total organic carbon was determined in samples at exposure initiation, at renewal, and at exposure termination since the test substance is a multi-constituent. At exposure initiation and at renewal from test item concentration and the control, two samples were collected for chemical analysis (each in a volume of 100 mL). One was transferred to chemical analyses as a fresh sample (at exposure initiation and at renewal), the other was stored under test conditions and transferred for chemical analyses as 24-hour old sample (at renewal and at exposure termination).

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTIONS
Appropriate amount of the test item was weighed in glass flask with ISO test water and mixed with appropriate volume of the ISO test water. The mixture of a loading of 100 mg/L was heterogeneous (with undissolved particles). The mixture and the control (800 mL ISO test water) were left for mechanical shaking for 48 hours (90 revolutions per minute, darkness). After 48 hours of mechanical shaking, the mixture was heterogeneous. The mixture and the control were filtrated, first through a conditioned paper filter (qualitative cellulose filter) and next through a conditioned nitrocellulose membrane filter (0.22 Xm, Millipore). Each filtrate was visually homogeneous (without any visible undissolved particles), clear, colourless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology

CULTURING
Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel) containing 100 mL of ISO test water at room temperature with daily cycle 16 h light : 8 h dark (KANLUX electronic time programmer, Poland). The culture was maintained in the ISO test water. The Daphnia magna were fed with a suspension of algae; mixture of two species Pseudokirchneriella subcapitata: Desmodesmus subspicatus (in 2:1 ratio) originating from separate cultures in the Laboratory of Aquatic Toxicology. Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilised suspension of Spirulina sp.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg/L as CaCO3

Test temperature:

19 - 22 °C

pH:

- Test start: 7.58 - 7.69
- Test termination: 7.55 - 7.69

Dissolved oxygen:

- Test start: 8.9 - 9.9 mg/L
- Test termination: 8.7 - 9.8 mg/L

Conductivity:

594 µS/cm

Nominal and measured concentrations:

- Nominal loading rates: 0 (control) and 100 mg/L (based on a range-finding test)
- Measured concentrations at study initiation (mg carbon/L): see 'Any other information on materials and methods incl. tables'.

Details on test conditions:

TEST SYSTEM
- Test vessel: 150 mL glass beakers
- Type: Closed, the beakers were covered with transparent lids in order to minimize evaporation and to prevent accidental contamination.
- Fill volume: 100 mL
- Renewal rate of test solution: Once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
- Age: less than 24 hours old (not first brood progeny)
- Feeding during test: no

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The ISO test water recommended by OECD Guideline No. 202 (2004) was used as diluent/solvent of the test item.
- Alkalinity: 66 mg/L as NaHCO3
- Culture medium different from test medium: No
- Intervals of water quality measurement (pH, dissolved oxygen): At start, after 24 and 48 hours. Temperature was measured continuously.

OTHER TEST CONDITIONS
- Photoperiod: 16 h : 8 h
- Lightning: fluorescent

EFFECT PARAMETERS MEASURED: immobilisation, determined after 24 and 48 hours exposure.

PRELIMINARY NON-GLP TEST
- Test loading rates: 0 (control), 1 (diluted), 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: no immobility was observed in the highest test concentration.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Details on results:

No immobility was observed up to the highest tested concentration. The fresh and 24-hour old test item concentration and the control were homogeneous, clear and colourless.

Results with reference substance (positive control):

The test with the reference material potassium dichromate was performed from March 01, 2017 to March 03, 2017.
- 48-h EC50: 0.80 mg/L (95% CI: 0.70 - 0.91 mg/L)
- 48-h EC20: 0.67 mg/L (95% CI: 0.54 – 0.75 mg/L)
- 48-h EC10: 0.61 mg/L (95% CI: 0.46 – 0.69 mg/L)
- 48-h LOEC: 1.00 mg/L
- 48-h NOEC: 0.56 mg/L

Reported statistics and error estimates:

No statistical analysis was needed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

See 'Any other information on materials and methods incl. tables'

Conclusions:

The test substance is with high probability not acutely harmful to aquatic invertebrates.