Sodium cumenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

EPA OTS 797.1400 (Fish Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

Test Substance
CAS Number: 28348-53-0
Identity: Cumene sulfonic acid, sodium salt
Purity: 45%
Remarks: Substances tested are aqueous solutions; % purity equates to chemical content

Analytical monitoring:

no

Details on sampling:

Statistical method: Binominal, moving average and probit analysis.
Number of fish: 10/test vessel, 2 test vessels/treatment.
Concentrations: Nominal: 100, 180, 320, 560 and 1000 mg/L (no vehicle);
untreated controls. Nominal concentrations have not been corrected for
sample purity.

Vehicle:

no

Details on test solutions:

Test conditions: Static without aeration; at 22 ± 1 °C in 19 L glass vessels containing 15 L of medium of hardness 140 mg/L (as CaCO3) and pH 8.2; 16
hours light; unfed.
Phys. meas.: At 0, 48 and 96 hours: overall ranges for pH 8.0-8.3; O2 >60%; temperature 22-23 °C.
Observations: Mortality/symptoms at 24, 48, 72 and 96 hours.
Minor remark. Fish were not fed for 72 hours prior to test initiation rather than 24 hours as stated in OECD 203

Test organisms (species):

Pimephales promelas

Details on test organisms:

Species: Pimephales promelas, mean length 23 ± 2 mm
Number of fish: 10/test vessel, 2 test vessels/treatment.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

140 mg/L (as CaCO3)

Test temperature:

22 ± 1 °C

pH:

8.2

Dissolved oxygen:

O2 >60%;

Details on test conditions:

Test conditions: Static without aeration; at 22 ± 1 °C in 19 L glass vessels
containing 15 L of medium of hardness 140 mg/L (as CaCO3) and pH 8.2; 16
hours light; unfed.
Phys. meas.: At 0, 48 and 96 hours: overall ranges for pH 8.0-8.3; O2 >60%;
temperature 22-23 °C.
Observations: Mortality/symptoms at 24, 48, 72 and 96 hours
Minor remark. Fish were not fed for 72 hours prior to test initiation rather than 24 hours as stated in OECD 203

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 450 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: 96-hr LC50 > 1000 mg/L (equivalent to >450 mg/L active ingredient taking into account the 45% purity of the sample).

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 96-hr LC50 > 1000 mg/L (equivalent to >450 mg/L active ingredient taking into account the 45% purity of the sample).

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

560 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Unit: mg/L

		Nominal Concentration (mg/L)
Parameter	Time [hour]	0	100	180	320	560	1000
Mortality [%]	96	None
Symptoms*	0-96						+

* Symptoms including surfacing and quiescence observed as indicated (+).

96-hr LC50 > 1000 mg/L (equivalent to >450 mg/L active ingredient taking into account

the 45% purity of the sample).

NOEC = 560 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 96-hr LC50 for Pimephales promelas, exposed to the test substance in a static system was >1000 mg/L (equivalent to >450 mg/L active ingredient taking into account the 45% purity of the sample).
NOEC = 560 mg/L

Description of key information

The 96-hr LC50 for Pimephales promelas, exposed to the test substance in a static system was >1000 mg/L (equivalent to >450 mg/L active ingredient taking into account the 45% purity of the sample).

NOEC = 560 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

450 mg/L

Marine water fish

Marine water fish

Effect concentration:

45 mg/L

Additional information

The available data for saltwater fishes a are limited and to obtaine the value for PNECs for saltwaters is taken account a assesment factor 10 to extrapolate to LC50 following the “Guidance on Information Requirement and Chemical Safety Assessment R.10: Characterisation of dose [concentration]- response for environment”and “Table R.10-5 Assessment factors proposed for deriving PNEC water for saltwater for different data sets”, to account for the possibility that greater taxonomic diversity in the marine environment makes it likely that some marine taxa may be more sensitive than the most sensitive freshwater taxa