4-[fluoro(dimethyl)silyl]butanenitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 12, 2016 - March 22, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 17. July 1992 “Fish, Acute Toxicity Test“

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-[fluoro(dimethyl)silyl]butanenitrile

EC Number:

812-925-4

Cas Number:

1639345-42-8

Molecular formula:

C6H12FNSi

IUPAC Name:

4-[fluoro(dimethyl)silyl]butanenitrile

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water-accommodated fractions were prepared for the test. This was done by weighing the nominal loads, adding the corresponding amount of dilution water and shaking vigorously
for 24 hours in the first experiment respectively 23 hours in the second experiment. experiment.
The resulting solutions were filtrated through 0.45 μm Cellulose ester filters.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Strain: Danio rerio HAMILTON-BUCHANAN
- Source: Dehner Garten Center, 67433 Neustadt
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): 2 +/- 1 cm

ACCLIMATION
- Acclimation period: at least 12 days
- Type and amount of food during acclimation: three times a day with warmwater fishfood , totalling about 1-2% of body weight per day


Husbandry
-Vessels: polyethylene aquaria
-Medium: chlorine-free tap water
-Photo period: 12/12 hours, using neon tubes
-Temperature: 23 +/- 2 °C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

0.83 mmol/L

Test temperature:

experiment 1: 20.5 – 21.8 °C
experiment 2: 20.4– 21.2 °C

pH:

experiment 1: 6.7 - 7.7
experiment 2: 6.5 -7.8

Dissolved oxygen:

The concentration of dissolved oxygen was at least 8.5 mg/L or 98 % in the first experiment respectively 7.7 mg/L or 85 % in the second experiment throughout the test.

Salinity:

not applicable

Conductivity:

228 µS/cm at 25°C

Nominal and measured concentrations:

The measured concentrations were in a range of 98 % - 104 % of the nominal concentration in the 1st experiment
and between 82 % - 104 % of the nominal concentration in the 2nd experiment. Therefore, the determination of the results of was based on the nominal concentrations.

Details on test conditions:

TEST SYSTEM

- Test vessel: glass aquaria, maximal volume 10 L

- Aeration: Yes

- Renewal rate of test solution (frequency/flow rate): static test

- No. of organisms per vessel: 7

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1



TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Good quality drinking water is used. The composition is analysed yearly by the supplier.



OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 12/12 hours using neon tubes


EFFECT PARAMETERS MEASURED: Mortality. After 24, 48, 72 and 96 h.


TEST CONCENTRATIONS

Experiment 1: 100 mg/L
Experiment 2: 10, 18, 32, 56, 100 mg/L

- Range finding study: no

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

94 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis products

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control:
Experiment 1: 1 out of seven
Experiment 2: 0 out of seven

Reported statistics and error estimates:

95% confidence interval was not determined due to mathematical reasons or inappropriate data.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Two experiments were performed.
The 1st experiment was performed as a limit test at the concentration 100 mg/L. Because significant mortality (57%) was observed in the treatment, a second experiment was performed using concentrations in the range of 10 – 100 mg/L.
In the 2nd experiment, only the highest concentrated treatment containing 100 mg/L showed 71% mortality.
A 96-hour LC50 value of 94 mg/L has been determined for the effects of the test substance on mortality of Danio rerio based on nominal exposure concentrations of the substance. The test substance hydrolyses in water and it is therefore likely that the test organisms were primarily exposed to the hydrolysis products of the substance.