2-amino-2-methylpropane-1,3-diol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1955

Report date:

1955

Materials and methods

Principles of method if other than guideline:

To estimate the LD50 value, mice were administered the test substance intraperitoneally and kept for observation for 7 days. A solution of the test substance was prepared on a weight/volume basis. The concentrations were varied to allow administration of a constant volume of 0.015 mL/g bw.

GLP compliance:

no

Remarks:

performed prior to GLP

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2-methylpropane-1,3-diol

EC Number:

204-100-7

EC Name:

2-amino-2-methylpropane-1,3-diol

Cas Number:

115-69-5

Molecular formula:

C4H11NO2

IUPAC Name:

2-amino-2-methylpropane-1,3-diol

Details on test material:

- Name of test material (as cited in study report): R-9
- Lot/batch No.: 396658R-9

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 15 g, on average

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: A solution of the test substance with an unknown vehicle was prepared

Details on exposure:

A solution of the test substance was prepared on a weight/volume basis. The concentrations were varied to allow administration of a constant volume of 0.015 mL/g bw.

Doses:

100, 250, 500, 1000 and 2000 mg/kg bw

No. of animals per sex per dose:

10 per dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days

Statistics:

The approximate LD50 doses were estimated by the method of Miller and Tainter, Proc. Soc. Exper. Biol. and Med., 1944; 57: 261.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

400 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Range: 400 ± 40 mg/kg bw

Mortality:

In the 100, 250, 500, 1000 and 2000 mg/kg bw dose groups, the mortality was 0, 1, 7, 9 and 10, respectively (see table 1).

Any other information on results incl. tables

Table 1: Mortality per dose group administered the test substance

Dose (mg/kg)	Number of animals treated	Mortality
100	10	0/10
250	10	1/10
500	10	7/10
1000	10	9/10
2000	10	10/10