Carbamic acid,N,N'-[1,3-phenylenebis[methyleneiminocarbonylimino(methyl-3,1-phenylene)]]bis-di-C11-14-isoalkylesters, C13-rich

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

bis(11-methyldodecyl) ((((1,3-phenylenebis(methylene)bis(azanediyl))bis(carbonyl) bis(azanediyl))bis(4-methyl-3,1-phenylene) dicarbamate

Cas Number:

865536-03-4

Molecular formula:

C52H80N6O6

IUPAC Name:

bis(11-methyldodecyl) ((((1,3-phenylenebis(methylene)bis(azanediyl))bis(carbonyl) bis(azanediyl))bis(4-methyl-3,1-phenylene) dicarbamate

Constituent 2

Reference substance name:

bis(11-methyldodecyl)(4-methyl-1,3-phenylene)dicarbamate

Molecular formula:

C35H62N2O4

IUPAC Name:

bis(11-methyldodecyl)(4-methyl-1,3-phenylene)dicarbamate

Constituent 3

Reference substance name:

11-methyldodecyl (3-(3-(3-(aminomethyl)benzyl)ureido)-4-methylphenyl)carbamate

Molecular formula:

C30H46N4O3

IUPAC Name:

11-methyldodecyl (3-(3-(3-(aminomethyl)benzyl)ureido)-4-methylphenyl)carbamate

Constituent 4

Reference substance name:

1,1'-(4-methyl-1,3-phenylene)bis(3-(3-(aminomethyl)benzyl)urea)

Molecular formula:

C25H30N6O2

IUPAC Name:

1,1'-(4-methyl-1,3-phenylene)bis(3-(3-(aminomethyl)benzyl)urea)

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Samples of secondary effluent were obtained as the test inoculum from a sewage plant treating predominantly domestic sewage (Nanjing Chengdong Sewage Treatment Plant) Aerobic conditions were maintained in the effluent by aeration from a laboratory supply of oil-free compressed air. (Batch No: 120120920) On the day of the test, the test inoculum was prepared by filtering the sample of sewage effluent through a coarse rilter. (discarding the first 200 mL) and collecting the remaining filtrates. This inoculum was maintained under aerobic conditions in the test area until use.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 60 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 1.41

Sampling time:

28 d

Details on results:

During the test, the temperature was kept between 19.6°C to 21.9°C. The test is valid because the biodegradation of the reference substance achieved 71.9% within 14 days, which was great than 60%, and biodegradation of the toxicity control was 26.7% within 14 days, which was great than 25%. The test substance did not inhibit the degradation of the reference substance and was therefore not toxic to the inoculum. Oxygen consumption in inoculum blanks was 1.21 mg O2/litre after 28-day test, which was less than 1.5 mg O2/L. Oxygen concentration in "test' was 7.41 mg O2/L after 28-day test, which was great than 0.5 mg O2/L. Therefore the test would be considered valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The result indicated the biodegradation rate of the test substance was 1.41% after 28 days, which indicated that Addukt TI80-MXDA is not readily biodegradable.

Executive summary:

The study was conducted according to the guidelines of SEPA HJ/T 153, "the guidelinesfor the testing of chemicals"; and with the reference to Part C.4-E (Closed Bottle Test) of EC Methods for the Determination of Ecotoxicity; Annex to Council Regulation (EC), L142 (2008); OECD Procedure 301D; Closed Bottle test and US Environmental Protection Agency (EPA), Office of Prevention, Pesticides and Toxic Substances, (OPPTS) Method 835.3110 (o), adopted January 1998; GB/T 21831-2008, Chemicals-Ready biodegradability; Closed bottle test. Under valid conditions, the ready biodegradability of Addukt TI80-MXDA was determined in a 28-day dissolve oxygen depletion using secondary effluent from a domestic waste water treatment plant.

During the test, the temperature was kept at (20士2) °C. The test is valid because the level of biodegradation of the reference substance reached 71.9% within 14 days, which was great than 60%, and biodegradation of the toxicity control was 26.9% within 14 days, which was great than 25%. The test substance did not inhibit the degradation of the reference substance and was therefore not toxic to the inoculum. Oxygen consumption in inoculum blanks was 1.21 mg O₂/litre after 28-day test, which was less than 1.5 mg O₂/L.

Oxygen concentration in "test" was 7.41 mg O₂/L after 28-day test, which was great than 0.5 mg O₂/L. Thus the study met the acceptability criteria prescribed by the protocol and was considered valid.

Finally the results showed that under valid conditions, the biodegradation rate of Addukt TI80-MXDA was 1.41% biodegradation after 28-days.