Registration Dossier
Registration Dossier
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EC number: 421-720-5 | CAS number: 18085-02-4 3,4-DIACETOXY-1-BUTENE
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data migrated from NONS with permission to refer granted by ECHA.
Data source
Reference
- Reference Type:
- other:
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 92/69/EEC (Ames)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2-diacetoxybut-3-ene
- EC Number:
- 421-720-5
- EC Name:
- 1,2-diacetoxybut-3-ene
- Cas Number:
- 18085-02-4
- Molecular formula:
- C8H12O4
- IUPAC Name:
- 1-(acetyloxy)but-3-en-2-yl acetate
- Test material form:
- liquid
- Details on test material:
- No information on test material available. Endpoint study information provided by ECHA from migrated NONS data and specific information not included.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Acrolor-induced rat liver S9
- Test concentrations with justification for top dose:
- Concentration range in the main test (with metabolic activation): 10...5000 µg/plate
Concentration range in the main test (without metabolic activation): 10...5000 µg/plate - Vehicle / solvent:
- Solvent: Dimethylsulfoxide
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: As specified above
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >/= 5000 µg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- other: As specified above
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- > 5000 µg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- other: As specified above
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >/= 5000 µg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Observations:
The solvent control plates gave counts in the expected ranges. All positive controls produced marked increases in the revertant counts. - Remarks on result:
- other: Preliminary test
Applicant's summary and conclusion
- Conclusions:
- Negative with and without metabolic activation.
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