9-[2-(2-methoxyethoxy)ethoxy]-9-[3-(oxiranylmethoxy)propyl]-2,5,8,10,13,16-hexaoxa-9-silaheptadecane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

4 March 1996 to 26 April 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

A 96-hour Acute toxicity of 9-[2-(2-methoxyethoxy)ethoxy]-9-[3(oxiranylmethoxy)propyl]- 2,5,8,10,13,16-hexaoxa-9-silaheptadecane to the common carp was determined in an test conducted in accordance with OECD 203, the information is reported to an appropriate standard but it is not clear if the study was conducted in accordance with Good Laboratory Practices.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

not specified

Specific details on test material used for the study:

Batch number: 84246753
Expiry date: 14 August 1997

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

The test solutions were prepared directly in ISO-medium. The final test solutions ranged from clear without precipitation to slightly turbid suspensions at 3,200 and 10,000 mg/1.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

seven fish per concentration and controls with exposed, loading was < 1.0 g fish/litre, i.e. 7 fish per 3 litres of test medium. The mean length and weight of the fish were 2.1 cm and 0.23 g respectively.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

250 mg CaCO3/L

Test temperature:

20.0 to 20.6°C

pH:

8.0

Dissolved oxygen:

8.8 - 9.3 mg/L

Salinity:

Not applicable

Conductivity:

Not applicable

Nominal and measured concentrations:

A range-finding test was performed to provide information about the range of concentrations to be used in the final test: three fish per concentration were exposed to a concentration range of 0.1 to 10 mg/l with an increasing factor of 10, while seven fish were exposed
to 0 and 100 mg/l.

During the Definitive test carp were exposed to concentrations exceeding 100 mg/l, i.e. ranging from 320 to 10,000 mg/l.

Details on test conditions:

Seven fish per concentration and controls were exposed to nominal concentrations of 9-[2-(2-methoxyethoxy)ethoxy]-9-[3(oxiranylmethoxy)propyl]- 2,5,8,10,13,16-hexaoxa-9-silaheptadecane in ISO-medium, aerated until the dissolved oxygen concentration had reached saturation and the pH had stabilized. After aeration the hardness was 250 mg CaC03 per litre and the pH was 8.0 + 0.2. Test vessels were all glass 6 litre tanks and the test media was aerated continuously with a 16 hour photoperiod. Test animals were not fed from 24 hours prior to the test and during the total test period. Fish were introduced to the test system direcly after preparation of test media.

The test solutions were prepared (320, 1000, 10000 mg/L) directly in ISO-medium. The final test solutions ranged from clear without precipitation to slightly turbid suspensions at 3,200 and 10,000 mg/L. Mortality and other effects At (2.5), 24, 48, 72 and 96 hours following the start of exposure. Dead fish were removed when observed. Dissolved oxygen content, pH and Temperature were measured daily from the start of the test.

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 96h-LC50 for carp is estimated at 4,200 mg/l with 0 % mortality at 1000 mg/l and 100 % mortality at 10,000 mg/l.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Linear extrapolation

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 for carp was estimated at 4,200 mg/l with 0 % mortality at 1000 mg/1 and 100 % mortality at 10,000 mg/l.

Executive summary:

A 96-Hour Acute Toxicity Study in Carp with9-[2-(2-methoxyethoxy)ethoxy]-9-[3(oxiranylmethoxy)propyl]- 2,5,8,10,13,16-hexaoxa-9-silaheptadecane was conducted.

The study procedures described in this report were based on the EEC directive 92/69, Part C.l. "Acute toxicity for fish"; and the OECD guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992.

The first toxicity test was a range-finding test at test substance concentrations of 0.1, 1.0, 10 and 100 mg/1. No effects were observed at any of these test concentrations. Then, a definitive test was performed with carp exposed to concentrations ranging from 320 to 10,000 mg/1 in a static system.

The 96h-LC50 for carp was estimated at 4,200 mg/l with 0 % mortality at 1000 mg/l and 100 % mortality at 10,000 mg/1.

Description of key information

A 96 -hour fish acute toxicity study was conducted in accordance with OECD203 using the rainbow trout, The 96h-LC50 was estimated to be >47 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

47 mg/L