4-hydroxy-6-(3-sulphoanilino)naphthalene-2-sulphonic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data from secondary source

Data source

Reference

Reference 1

Reference Type:

other: Authoritative database

Title:

Acute dermal toxicity for CAS NO: 107-64-2

Author:

IFA GESTIS

Year:

2017

Bibliographic source:

GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn), 2017

Reference 2

Reference Type:

secondary source

Title:

Initial Submission: Letter from Monsanto Co to USEPA regarding Cyanuric Acid Crude with Attachments and Cover Letter Dated 072392

Author:

NTRL

Year:

1992

Bibliographic source:

NTRL report, OTS0538650, 1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

An acute dermal toxicity study of Cyanuric acid was carried out on rabbits

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyanuric acid

EC Number:

203-618-0

EC Name:

Cyanuric acid

Cas Number:

108-80-5

Molecular formula:

C3H3N3O3

IUPAC Name:

1,3,5-Triazin-2,4,6-triol

Details on test material:

- Name of test material: Cyanuric acid
- IUPAC name: 1,3,5-Triazinane-2,4,6-trione
- Molecular formula: C3H3N3O3
- Molecular weight: 129.075 g/mol
- Substance type: Organic

Specific details on test material used for the study:

- Name of test material: Cyanuric acid
- IUPAC name: 1,3,5-Triazinane-2,4,6-trione
- Molecular formula: C3H3N3O3
- Molecular weight: 129.075 g/mol
- Substance type: Organic

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

Details on test animal
TEST ANIMALS
- Source: Isf:(NZW), Isaac's Farm, Litchfield, Illinois)
- Age at study initiation:
- Weight at study initiation:2.51-2.79kg
- Fasting period before study: No data available
- Housing: Each animal was identified by ear tag and bar coded cage card.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To:

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

DOSAGE PREPARATION (if unusual): The test material, ground with a mortar and pestle and moistened with physiological saline,
TEST SITE
- Area of exposure: Dorsal surface
- % coverage: no data
- Type of wrap if used: occlusive wrap of latex rubber
secured by bandaging and elastic tape


REMOVAL OF TEST SUBSTANCE
- Washing (if done): material was wiped from the animal.
- Time after start of exposure:24hr

TEST MATERIAL
- Amount(s) applied (volume or weight with unit):5000mg/kg bw
- Concentration (if solution): No data available
- Constant volume or concentration used: yes/no
- For solids, paste formed: yes,The test material, ground with a mortar and
pestle and moistened with physiological saline


VEHICLE
- Amount(s) applied (volume or weight with unit):No data available
- Concentration (if solution):No data available
- Lot/batch no. (if required): No data available
- Purity:No data available

Duration of exposure:

24hr(test material was held in place by means of an occlusive wrap of latex rubber secured by bandaging and elastic tape. The occlusive wrap was removed after approximately 24 hours and the excess material was wiped from the animal.)

Doses:

5000mg/kg

No. of animals per sex per dose:

5 male and 5 female

Control animals:

not specified

Details on study design:

Details on study design
- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: Clinical observations were made three times during the first eight hours following test
material administration and twice daily (morning and afternoon) thereafter, with the exceptions of one day when observations were only recorded once and another day when
Observations were only recorded once for nine of the animals. Body weights were recorded on days a (day of exposure), 7, and 14.

- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, were done
other:

Statistics:

No data available

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality was observed

Mortality:

No mortality was observed

Clinical signs:

other: No clinical abnormalities were observed.

Gross pathology:

At necropsy, one male animal had pale colored kidneys, one female animal had kidneys with pale, mottled exteriors, and another female had tapeworm cysts in the mesentery.None of these effects were attributed to toxicity of the test material.

Other findings:

No data available

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Conclusions:

The LD 50 was considered to be >5000 mg/kg .When rabbits treated with Cyanuric acid (108-80-5) by dermal application.

Executive summary:

An acute dermal toxicity study of Cyanuric acid(108-80-5) was carried out on New Zealand White rabbits. A group of 5 male and 5 female rabbits were given a single dose of 5000mg/kg bw test materialground with a mortar and pestle and moistened with physiological saline, on the dorsal Surface. Before application the skin on the dorsal surface of each animal was shaved with an electric clipper and abraded with a hypodermic needle.Test materialwas held in place by means of an occlusive wrap of latex rubber secured by bandaging and elastic tape. The occlusive wrap was removed after approximately 24 hoursand the excess material was wiped from the animal. Clinical observations were made three times during the first eight hours following test material administration and twice daily (morning and afternoon) thereafter, with the exceptions of one day when observations were only recorded once and another day when observations were only recorded once for nine of the animals. Body weights were recorded on days a (day of exposure), 7, and 14.No mortality was observed at dose 5000mg/kg bw.At necropsy, one male animal had pale colored kidneys, one female animal had kidneys with pale, mottled exteriors, and another female had tapeworm cysts in the mesentery. None of these effects were attributed to toxicity of the test material. Also no clinical abnormalities were observed.Therefore LD 50 was considered to be >5000 mg/kg .When rabbits treated with Cyanuric acid(108-80-5) by dermal application.