1-chlorooctane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(1992)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

A sample of the test solution was taken from each vessel at the start of the test and after 24 h.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: addition of 500 mg test substance per liter dilution water
- Controls: control group with dilution water only, vehicle control with 10 mg/L vehicle
- Chemical name of vehicle: HCO-50
- Concentration of vehicle in test medium: 10 mg/L
- Evidence of undissolved material: In the highest concentration a slight white mudiness occured, but no precipitations were observed.

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish
- Strain: Oryzias laptides
- Source: Rokuotsu Hatchery

- Length at study initiation (length definition, mean, range and SD): 2.26 cm
- Weight at study initiation (mean and range, SD): 0.1552 g
- Feeding during test: feeding was stopped 1 d before the test

ACCLIMATION
- Acclimation period: 12 d
- Acclimation conditions: same as test conditions
- Health during acclimation (any mortality observed): healthy and normal animals were used for the test

Study design

Test type:

semi-static

Limit test:

no

Total exposure duration:

96 d

Test conditions

Hardness:

30 mg/l CaCO3

Test temperature:

23.3 - 24.2 °C

pH:

pH=6.8 -7.1

Dissolved oxygen:

5.4 - 10.5 mg/L, 60% or more than the satured oxygen concentration

Nominal and measured concentrations:

nominal: 0.95 mg/L, 1.71 mg/L, 3.09 mg/L, 5.56 mg/L, 10.00 mg/L
measured: 0.22 mg/L, 0.29 mg/L, 0.49 mg/L, 1.24 mg/L, 4.26 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessel (3L volume)
- Material, size, headspace, fill volume: glass vessel filled with 3 L test solution
- Aeration: none
- Type of flow-through: semi-static
- Renewal rate of test solution (frequency/flow rate): every 24 h renewal of medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was Nagoya municipal water which was de-chlorinated by acitvated chocoal.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light and 8 h darkness
- Light intensity: room light

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: preliminary test
- Range finding study: A preliminary test resulted in a LC50 of 4.1 mg/L.
- Test concentrations: 0.95 mg/L, 1.71 mg/L, 3.09 mg/L, 5.56 mg/L, 10.00 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

copper(II)sulfate pentahydrate

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.62 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confindence range 0.41 mg/L - 0.92 mg/L

Details on results:

- Behavioural abnormalities: abnormal respiration and abnormal swimming behaviour with 0.29 mg/L and 0.49 mg/L, abnormal swimming with 1.24 mg/L
- Mortality of control: none
- Other adverse effects control: none

LC10=0.22 mg/L
LC100= 4.26 mg/L

Results with reference substance (positive control):

Copper(II)sulfate pentahydrate: 96 hour LC50 of 0.73 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test of Oryzias latipes with 1-chlorooctane resulted in a LC50 of 0.62 mg/L after 96 h.