Registration Dossier
Registration Dossier
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EC number: 204-844-2 | CAS number: 127-47-9
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication which meets basic scientific principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
- Principles of method if other than guideline:
- Acute toxicity after intraperitoneal injection in mouse
- GLP compliance:
- no
Test material
- Reference substance name:
- Retinyl acetate
- EC Number:
- 204-844-2
- EC Name:
- Retinyl acetate
- Cas Number:
- 127-47-9
- Molecular formula:
- C22H32O2
- IUPAC Name:
- (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-yl acetate
- Details on test material:
- Vitamin A acetate (CAS No. 127-47-9) in arachis oil; according to the authors, purity was 1.53 mio IU/g (ca. 530 mg Vitamin A acetate/g or 53%); no further data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- olive oil
- Doses:
- 1780, 2150, 2610, 3160, 3830, 5620, 8250 mg/kg bw
- No. of animals per sex per dose:
- 5-10
- Control animals:
- no
- Details on study design:
- Groups of male and female NMRI mice were given a single intraperitoneal
dose of the test substance (as a 70% (w/v) suspension in olive oil) and were
observed for 14 days. Examinations included mortality (after 1, 24, and
48 hour(s), and after 7 and 14 days, signs of toxicity, and necropsy of
decedents and survivors sacrificed at the end of the observation period. - Statistics:
- Probit Analysis.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 390 mg/kg bw
- Mortality:
- see table
- Clinical signs:
- Signs of toxicity were observed at all dose levels and comprised dyspnea, intermittent respiration, apathy, anomal position, staggering, tremors, twitching, spastic gait, stretching, piloerection, exsiccosis, encrustations at the snout and eyes, paresis, and poor general state.
- Body weight:
- There was no statistically significant effect on body weight.
- Gross pathology:
- Pathological examination revealed no findings.
Any other information on results incl. tables
Mortality after 14 days:
1780 mg/kg bw: 2/10 males; 1/10 females
2150 mg/kg bw: 4/10 males; 1/10 females
2610 mg/kg bw: 6/10 males; 8/10 females
3160 mg/kg bw: 9/10 males; 8/10 females
3830 mg/kg bw: 5/5 males; 5/5 females
5620 mg/kg bw: 5/5 males; 5/5 females
8250 mg/kg bw: 5/5 males; 5/5 females
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
