4-methyl-1-phenyl-3-pyrazolidone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of microorganism for the test chemical :
2. Effective concentration was determined for Photobacterium phosphoreum using Microtox test.
3. Preliminary, determination of the inhibitory effect of wastewater containing substance on the Oxygen consumption of Pseudomonas putida.

Analytical monitoring:

not specified

Test organisms (species):

other: 2.Photobacterium phosphoreum 3.Pseudomonas putida

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

15 degree C

pH:

5-7

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

3.02 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: viability and sensitivity of the bacteria

Remarks on result:

other: no other information

Dose descriptor:

EC50

Effect conc.:

10 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: no other information

Conclusions:

The test chemical is not likely to be toxic to microorganism in the dose range of 3.02 to 10000 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

Photobacterium phosphoreum was test organism used to determined the toxicity of test material done by Microtox test method . The time period was 5 to 30 minute at 15 degree C and pH range from 5 to 9. After exposure, the 50% Effective concentration value for 1 test chemical to Photobacterium phosphoreum was determined to be 3.02 mg/l based on viability and sensitivity of bacteria.

In another study for structurally similar read across substance , Pseudomonas respiratory arrest test was carried out for the test material substance.The 50% Effective concentration (EC50) of the substance5is determined to be >10000 mg/L based on inhibitory effect on Oxygen consumption (respiration).

Description of key information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

Photobacterium phosphoreum was test organism used to determined the toxicity of test material done by Microtox test method . The time period was 5 to 30 minute at 15 degree C and pH range from 5 to 9. After exposure, the 50% Effective concentration value for 1 test chemical to Photobacterium phosphoreum was determined to be 3.02 mg/l based on viability and sensitivity of bacteria.

In another study for structurally similar read across substance , Pseudomonas respiratory arrest test was carried out for the test material substance.The 50% Effective concentration (EC50) of the substance5is determined to be >10000 mg/L based on inhibitory effect on Oxygen consumption (respiration).

Key value for chemical safety assessment

EC50 for microorganisms:

10 000 mg/L

Additional information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

Photobacterium phosphoreum was test organism used to determined the toxicity of test material done by Microtox test method . The time period was 5 to 30 minute at 15 degree C and pH range from 5 to 9. After exposure, the 50% Effective concentration value for 1 test chemical to Photobacterium phosphoreum was determined to be 3.02 mg/l based on viability and sensitivity of bacteria.

In another study for structurally similar read across substance , Pseudomonas respiratory arrest test was carried out for the test material substance.The 50% Effective concentration (EC50) of the substance5is determined to be >10000 mg/L based on inhibitory effect on Oxygen consumption (respiration).