Trisodium [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 15, 1994 to December 29, 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

April 4, 1984 (Edition : Paris, 1993).

Deviations:

yes

Remarks:

1. Preparation of the inoculum: higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines). 2. 4 instead of 5 test concentrations.

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT 20063/C
Batch-Nr.: 3.46
Purity: 81.9 %
Appearance: solid
Solubility: >10 g/l [in water]
Storage: room temperature
Expiration date: 04/99

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Stock solutions : 500 mg/l test substance or reference substance in deionized water.

Test organisms (species):

activated sludge

Details on inoculum:

- Name and location of sewage treatment plant where inoculum was collected: Communal sewage treatment plant (ARA-Pro Rheno Basel)

- Pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

pH:

6.9 before the test beginning (Sludge)

Nominal and measured concentrations:

320, 100, 32 and 10 mg/l of the test substance.
32, 10 and 3.2 mg/l of the reference substance.

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
Sludge dry weight: 1.38 g/l
Test volume: 100 ml
Test duration: 3h
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode
system: OXI DIGI 530 (SAV : 00 109)
Aeration: 0.1 - 0.2 l/min by pasteur-pipette
pH of sludge: 6.9 before the test beginning

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

The IC50 (3 h) of the reference substance 3,5-Dichlorophenol : 11 mg/l

None

Validity criteria fulfilled:

yes

Conclusions:

IC50 (3 h) of the test substance FAT 20063/C was determined to be >320 mg/l.

Executive summary:

The toxicity of FAT 20063/C to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Directive 87/302, L 133/118 -122 and to the OECD Guideline 209, in compliance with GLP. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. Based on the findings of the study, IC50 (3 h) of the test substance FAT 20063/C was determined to be >320 mg/l, while the IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 11 mg/l.

Description of key information

The 3 hour IC50 for the substance >300 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

300 mg/L

Additional information

Three studies are available for assessment of toxicity potential of FAT 20063 to bacterial population.

In a key study, the toxicity of FAT 20063/C to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Directive 87/302, L 133/118 -122 and to the OECD Guideline 209, in compliance with GLP. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. Based on the findings of the study, IC50 (3 h) of the test substance FAT 20063/C was determined to be >320 mg/l, while the IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 11 mg/l.

Two supporting studies conducted in 1975 and 1981, also support the conclusion that the IC50 for the substance is >300 mg/L.