Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

limited documentation regarding composition of test material

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate

EC Number:

265-529-3

EC Name:

Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate

Cas Number:

65151-26-0

Molecular formula:

C17H15N3O7S2.2Na

IUPAC Name:

disodium 7-[(4-amino-3-methoxyphenyl)diazenyl]naphthalene-1,3-disulfonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION

the test substance was added directly to the test medium (100 ± 0.5 mg/L)

Test organisms

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Guppy
- Length at study initiation (mean): 2.6 cm*
- Weight at study initiation (mean): 0.13 g*
*weight and length values refer to test organisms after termination of the test
- Method of breeding: parental and young fish population was held in two 250 L glass aquaria at the testing facility at 23 ± 2 °C, illumination 14 h/day. The tap water from Schlieren was continuously aerated and filtered . The bottom of the aquaria was covered with gravel. On third of the toal volume of the aquaria was replaced by fresh tap water monthly.
- Feeding: Sera micropan, Sera vipan daily (Sera apanristic GmbH, D-52518 Heinsberg, Germany). The test fish were last fed 24 h before the test started. No feeding during the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations were carried out during exposure at the beginning of the experiment and after 24, 48, 72 and 96 hours.

Test conditions

Test temperature:

20°C - 21°C (treatment and control)

pH:

8.1 - 8.7 (treatment), 8.2 - 8.8 (control)

Dissolved oxygen:

5.6 - 7.6 mg/L (treatment), 6.1 - 7.2 mg/L (control)

Conductivity:

510 - 585 µS/cm

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Glass-aquaria, about 1 L per vessel
- Aeration: aerated
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water from Schlieren.
- Culture medium different from test medium: no
- Intervals of water quality measurement:
pH , temperature and oxygen saturation: at the beginning and every 24 h in all vessels;


OTHER TEST CONDITIONS
- Photoperiod: 16 h per day, supplied by overhead white fluorescent tubes


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and behavior of the test fish and control fish were observed at 2, 24, 48, 72, 96 h.


TEST CONCENTRATIONS
- 100 mg/L, nominal

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 3

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Details on results:

- There was no mortality in control as well as in treated fish at any observation time point.
- Behavioural abnormalities: Neither in treated nor in control fish any abnormalities (e.g. loss of coordination, hypo- or hyperactivity and swimming on the back) could be observed at any observation time points.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this study the test item did not cause any effects to guppy after 96 h when tested with the nominal concentration of 100 mg/L.

Executive summary:

The acute toxicity of the test item to fish (guppy) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) . A static limit test with a 100 mg/L solution was conducted. Duration of the test was 96 h. 7 test organisms were exposed to the 100 mg/L solution and the control. Water quality parameters pH-value, temperature and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. No test item analysis was carried according to the sponsor.

The 100 mg/L solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 96 hours. Therefore, the NOEC and LC₀ were laid down as >= 100 mg/lL (based on the nominal concentration) and LC₁₀₀ was laid down as > 100 mg/L (based on the nominal concentration).