Benzenesulfonic acid, 4-chloro-2-[2-[4,5-dihydro-3-methyl-5-oxo-1-(3-sulfophenyl)-1H-pyrazol-4-yl]diazenyl]-5-methyl-, ammonium salt (1:2)

Biodegradation in soil

Some information in this page has been claimed confidential.

Administrative data

Endpoint:

biodegradation in soil: simulation testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1994-09-15 until 1994-10-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Anglian Water sewage treatment plant (Godmanchester), sample collected on 15 September 1994
- Preparation of inoculum for exposure: The sample was allowed to settle for approximately 30 minutes and the supernatant filtered through Whatman GFA coarse filter paper (first 250 ml discarded). The filtrate was maintained under aeration and used within 24 hours of collection.
- Pretreatment: none

Duration of test (contact time):

28 d

Initial conc.:

5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Solubilising agent (type and concentration if used): None
- Test temperature: 20 ± 1°C
- Other: Use of darkened glass bottles

TEST SYSTEM
- Culturing apparatus: The bottles were incubated in a water bath at 20 ± 1°C until removed for measurement
- Number of culture flasks/concentration: duplicate bottles at each sampling time
- Measuring equipment: Yellow Springs BOD Meter (Model 59)

SAMPLING
- Sampling frequency: At day 0, 4, 7, 11, 14, 18, 21, 25 and 28 (duplicate bottles at each sampling time)

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes ( 5 mg/l test substance plus 3 mg/l sodium benzoate)

Reference substance:

benzoic acid, sodium salt

Remarks:

3 mg/L

Parameter:

% degradation (O2 consumption)

Value:

8

Sampling time:

28 d

Details on results:

Cultures containing both test and standard substances combined showed an oxygen depletion value 12 % higher than that anticipated on the basi of results from separate cultures at Day 14. Consequentiy the test substance is not considered to have had an inhibitory effect on sewage bacteria under the conditions of this test.

Results with reference substance:

Sodium benzoate attained 77 % biodegradation within 28 days.

Validity criteria fulfilled:

yes

Interpretation of results:

other: Not readily biodegradable according to OECD criteria

Conclusions:

The test substance may not be termed as readily biodegradable.
There was no evidence of inhibitory effects under the conditions of this test.

Cross-reference 2

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline sudy with analytical monitoring

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The sludge collected from ten different places in Japan were combined and cultivated for one month
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Solubilising agent (type and concentration if used): no data
- Test temperature: 25 °C +/- 1°C

CONTROL AND BLANK SYSTEM
- Toxicity control: yes (Aniline)

Reference substance:

aniline

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Tab. 1: Percent biodegradability based on oxygen consumption

	Test substance		Reference substance (aniline)
	day	%	day	%
No. 1	28	-1	28	67
No. 2	28	0	(7)	(62)
No. 3	28	1

Tab. 2: Percent biodegradability based on HPLC analyses. The concentrations of the test substance in the test mediums after 28 days were determined by HPLC and biodegradabilities (%) calculated.

	Test substance
	day	concentration (mg/L)	biodegradability (%)
No. 1	28	100	0
No. 2	28	100	0
No. 3	28	100	0

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Cross-reference 3

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Study period:

2020

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification

Principles of method if other than guideline:

Estimation of ready biodegradation in water using CATALOGIC v5.14.1.5 BOD 28 days MITI (OECD 301C) v.11.16

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

other: Model calculation

Duration of test (contact time):

28 d

Parameter:

% degradation (O2 consumption)

Remarks:

QSAR model: based on OECD TG 301 C

Value:

1

Sampling time:

28 d

Remarks on result:

not readily biodegradable based on QSAR/QSPR prediction

Remarks:

within parameter domain, out of structural fragment domain, within metabolic domain

Details on results:

Predicted value (model result):
0.01 ± 5.68E-3
Concomitant predictions :
Not ready degradable
Primary Half Life = < 1 day
Ultimate Half Life = 7y 5m 7d

Interpretation of results:

not readily biodegradable

Conclusions:

The substance is not readily biodegradable. In total 18 metabolites at a quantity >=0.1% could be identified. None of these metabolites have logKow values indicative of a significant bioaccumulative potential.

Results and discussion

Transformation products:

not measured

Remarks:

relevant degradation products are not expected