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REACH

3-nitrobenzoic acid

EC number: 204-508-5 | CAS number: 121-92-6

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

Analytical monitoring:

not required

Remarks:

Stable under recomended conditions

Vehicle:

not specified

Details on test solutions:

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After stirring the stock was analytically detected and the solubility was found 937.84 mg/L.By considering this value calculations done for following concentrations 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Anytime Pet,Jaripatka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.8 cm
- Weight at study initiation (mean and range, SD): average 0.082 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

155 mg of CaCO3

Test temperature:

23.4 °C

pH:

7.4

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortality was observed

Details on results:

No mortality was observed

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal	Number of		Mortality (cumulated) after
Concentration fish			3 h		6 h		24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
6.25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
12.5	4	4	-	-	-	-	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

24h

48h

72h

96h

Control

NS4

6.25

NS4

12.5

NS4

100

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

8.2. pH and oxygen content:

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.5	7.5	7.6	7.6	7.8	7.8	7.9	7.9
6.25	4	4	7.7	7.7	7.8	7.8	7.9	7.9	8.0	8.0
12.5	4	4	7.7	7.7	7.8	7.8	7.9	7.9	8.0	8.0
25	4	4	7.8	7.8	7.8	7.8	7.9	7.9	8.1	8.1
50	4	4	7.9	7.9	7.9	8.0	8.2	8.2	8.3	8.3
100	4	4	8.0	8.0	8.1	8.1	8.2	8.2	8.3	8.3

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
6.25	4	4	6.8	6.8	6.8	6.8	6.6	6.6	6.5	6.5
12.5	4	4	6.8	6.8	6.8	6.8	6.6	6.6	6.5	6.5
25	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
50	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
100	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC0 (96 h)] for test material on Zebra Fish Danio rerio was determine to be 100 mg/l.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After stirring the stock was analytically detected and the solubility was found 937.84 mg/L.By considering this value calculations done for following concentrations 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to all nominal test concentrations, LC0 was determine to be 100 mg/l . Based on the LC0, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After stirring the stock was analytically detected and the solubility was found 937.84 mg/L.By considering this value calculations done for following concentrations 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to all nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 100 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After stirring the stock was analytically detected and the solubility was found 937.84 mg/L.By considering this value calculations done for following concentrations 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to all nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

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