3-methylsulphanilic acid

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Principles of method if other than guideline:

Biodegradation study was carried out for 28 days for evaluating the percentage biodegradability of the test substance 4-Amino-3-methylbenzenesulfonic acid. The study was performed according to OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test).

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 4-amino-3-methylbenzenesulfonic acid
- Molecular formula: C7H9NO3S
- Molecular weight: 187.218 g/mol
-Smiles: S(c1cc(c(N)cc1)C)(O)(=O)=O
- InChI: 1S/C7H9NO3S/c1-5-4-6(12(9,10)11)2-3-7(5)8/h2-4H,8H2,1H3,(H,9,10,11)
- Substance type: Organic
- Physical state: Solid

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

28 d

Based on:

not specified

Parameter followed for biodegradation estimation:

DOC removal

Key result

Parameter:

% degradation (DOC removal)

Value:

22

Sampling time:

28 d

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 22% degradation by DOC removal parameter in 28 days.

Table:Aerobic Degradation of Aromatic Amines (by Zahn-Wellens test).

 

 

Aromatic amine	Degradation period (days)	% degradation
		DOC analysis	Specific analysis
4-amino-3-methylbenzenesulphonic acid	28	22	-

Validity criteria fulfilled:

not specified

Interpretation of results:

inherently biodegradable

Conclusions:

The percentage degradation of test substance was determined to be 22% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 4-Amino-3-methylbenzenesulfonic acid was considered to be inherently biodegradable in water.

Executive summary:

Biodegradation study was carried out for 28 days for evaluating the percentage biodegradability of the test substance 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9). The study was performed according to OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) under aerobic conditions. The percentage degradation of test substance was determined to be 22% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 4 -Amino-3 -methylbenzenesulfonic acid was considered to be inherently biodegradable in nature.

Description of key information

Biodegradation study was carried out for 28 days for evaluating the percentage biodegradability of the test substance 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9). The study was performed according to OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) under aerobic conditions. The percentage degradation of test substance was determined to be 22% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 4 -Amino-3 -methylbenzenesulfonic acid was considered to be inherently biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Various study and predicted data for the target compound 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9) and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study (D. Brown and B. Hamburger, 1987), biodegradation experiment was carried out for 28 days for evaluating the percentage biodegradability of the test substance 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9). The study was performed according to OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) under aerobic conditions. The percentage degradation of test substance was determined to be 22% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 4 -Amino-3 -methylbenzenesulfonic acid was considered to be inherently biodegradable in nature.

 

In a supporting study for test substance 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9) from peer reviewed journal (D. Brown and B. Hamburger, 1987), biodegradation study was carried out for 28 days for evaluating the percentage biodegradability of the test substance 4 -Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9). The study was performed according to EPA OTS 796.3180 (Ready Biodegradability: Modified AFNOR Test) under aerobic conditions. The percentage degradation of test substance was determined to be 2 and 12% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 4 -Amino-3 -methylbenzenesulfonic acid was considered to be not readily biodegradable in water.

 

Another supporting biodegradation study (D. Brown and B. Hamburger, 1987) was carried out for evaluating the percentage biodegradability of the test substance 4 - Amino-3 -methylbenzenesulfonic acid (CAS no. 98 -33 -9). Activated sludge was used as an inoculum and the study was performed under anaerobic conditions at a temperature of 35°C for a period of approx. 100 days. Samples of the aqueous phase were analyzed either qualitatively or quantitatively by an appropriate chromatographic method for the presence of certain of the expected aromatic amine metabolites. The percentage degradation of test substance was determined to be 0% by appropriate chromatography method and DOC analysis in approx. 100 days. Thus, based on percentage degradation, 4 -Amino-3 -methylbenzenesulfonic acid was considered to be not readily biodegradable in nature.

 

Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound 4-Amino-3-methylbenzenesulfonic acid (CAS no. 98 -33 -9) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 4 -Amino-3 -methylbenzenesulfonic acid is expected to be not readily biodegradable.

For read across substance 2-amino-5-methylbenzene sulfonic acid (CAS no. 88 -44 -8) (J-CHECK, 2016), biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of substance 2-amino-5-methylbenzene sulfonic acid. Concentration of inoculum i.e, activated sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance was determined to be <0 and 0% by BOD, O2 consumption, TOC removal, Test mat. analysis and UV-Vis parameter in 14 days. Thus, based on percentage degradation, 2-amino-5-methylbenzene sulfonic acid is considered to be not readily biodegradable in water.

 

On the basis of above results for target chemical 4 -Amino-3 -methylbenzenesulfonic acid (from peer reviewed journal and EPI Suite) and read across substance from authortiative database (J-CHECK, 2016), it can be concluded that the test substance 4 -Amino-3 -methylbenzenesulfonic acid can be expected to be not readily biodegradable in nature.