Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-012-8 | CAS number: 16941-92-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- not stated
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Not to current international guidleines, with significant deviations from the protocol. For example no revertant data provided, no metabolic activation, and no controls used.
Data source
Reference
- Reference Type:
- publication
- Title:
- Rec assay and mutagenicity studies on metal compounds.
- Author:
- Kanematsu N, Hara M and Kada T
- Year:
- 1 980
- Bibliographic source:
- Mutation Research 77, 109-116.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The spot test, first described by Iyer and Sybalsky (1958) and later by Ames et al. (1975).
- GLP compliance:
- not specified
- Type of assay:
- other: Spot test
Test material
- Reference substance name:
- Hexachloroiridic acid
- EC Number:
- 241-012-8
- EC Name:
- Hexachloroiridic acid
- Cas Number:
- 16941-92-7
- Molecular formula:
- Cl6Ir.2H
- IUPAC Name:
- hexachloroiridium(2-);hydron
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): H2IrCl6 (name & CASRN not reported)
- Analytical purity: "Of the highest purity commercially available" in Japan.
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli, other: B/r WP2 try- and WP2 hcr- try-
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0.001-10 M
- Details on test system and experimental conditions:
- Bacterial strains were spread on plates containing broth-enriched agar. Filter-paper disks containing the test substance were placed onto the plates. Revertant colonies around the disk were counted. Reversion assays with the test substance in liquid were also carried out with the two E.coli strains.
Results and discussion
Test results
- Species / strain:
- other: all strains
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
In a limited assay (Spot test), hexachloroiridic acid failed to induce reverse mutations in bacteria. - Executive summary:
In a limited assay (the spot test), filter paper discs containing hexachloroiridic acid were place onto agar plates containing bacterial strains Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 or Escherichia coli strains B/r WP2 try- and WP2 hcr- try-. Revertant colonies around the discs were counted. Reversion assays with the test substance in liquid were also carried out with the two E.coli strains.
The test substance (at up to 10 M) failed to induce reverse mutations in bacteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
