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Diss Factsheets
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EC number: 200-774-1 | CAS number: 72-19-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- L-threonine
- EC Number:
- 200-774-1
- EC Name:
- L-threonine
- Cas Number:
- 72-19-5
- Molecular formula:
- C4H9NO3
- IUPAC Name:
- L-threonine
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: rat/CD/Crl:CD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: males 45 days, females 56 days
- Weight at study initiation: males 204-228 g, females 194-220 g
- Fasting period before study: 16 hours before exposure
- Housing: caged by sex in groups of 2-3 animals/cage
- Diet: feeding was discontinued approx. 16 hours before exposure
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C +/- 3°C
- Humidity (%): 55% +/- 15%
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 17.11.2003 To: 02.12.2003
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- nose only
- Vehicle:
- clean air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: dynamic inhalation chamber (Rotameter, ROTA Apparate- und Maschinenbau, 0-79664 Wehr/Baden)
- Exposure chamber volume: 40 L
- Method of holding animals in test chamber: in pyrex tubes at the edge of the chamber in a radial position.
- Flow rate of air: 900 L/h
- Method of conditioning air: air was taken from the surrounding atmosphere of the laboratory room and filtered using an in-li ne disposable
gas filter
- System of generating particulates/aerosols: rotating brush dust generator (RBG 1000, PALAS GmbH Partikel und Lasermesstechnik, 0-76229
Karlsruhe)
- Method of particle size determination: cascade impactor (MAY, K.R. Aerosol impaetion jets, J.Aerosol Sei..§, 403 (1975), RESEARCH ENGINEERS Ltd, London N1 5RO, UK
- Treatment of exhaust air: passage through appropriate filter
- Temperature, humidity, oxygen content in air chamber: temperature 22°C +/- 3°C, humidity based on the guidelines, recordings of humidity in
the chamber are not made where dust examinations are carried out, oxygen content 21%
TEST ATMOSPHERE
- Brief description of analytical method used: Dust sampies were taken once every hour during the exposure. For that purpose, a SARTORIUS filter
(Minisart SM 17598 0.45 µm) was placed in the purpose-made inlet of the inhalation chamber in such a way that the opening of the filter was close
to the animals' noses. By means of a piece of silicon tubing, the filter was connected to an air-flow meter attached to a pump. The filter was
weighed before and after sampling (accuracy: 10 µg)
- Samples taken from breathing zone: yes
VEHICLE
- Composition of vehicle: clean air
- Concentration of test material in vehicle:
- Purity: filtered using an in-line disposable gas filter)
TEST ATMOSPHERE
- MMAD (Mass median aerodynamic diameter): 2.383 µm
- Geometric mean diameter: 3.3575 µm
- GSD (Geometric st. dev.): 3.629 µm - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 5.15 +/- 0.10 mg/l
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations for mortality and clinical signs: during and after exposure, daily thereafter
- Frequency of weighing: day 0 (prior to dosing), day 7 and day 14
- Necropsy of survivors performed: yes
- Other examinations performed: gross examination of organs at necropsy, organ weights,
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC0
- Effect level:
- > 5.15 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5.15 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- no mortality occurred
- Clinical signs:
- other: Particular attention was directed to observation of tremor, convulsions, salivation, diarrhoea, lethargy, sleep and coma
- Body weight:
- Individual weights of animals were determined before the exposure and weekly after exposure. Changes in weight were calculated and recorded when survival exceeds one day.
- Gross pathology:
- Macroscopic post mortem findings: no pathological findings
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- Under the present test conditions, no signs of toxicity were observed at 5.15 ± 0.1 0 mg L-threonine/L air. No mortality occurred. No abnormalities were detected at necropsy. The animals gained the expected weight throughout the study period.
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