Ethyl 2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study in compliance with GLP-criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl trans-(±)-2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate hydrochloride

EC Number:

248-226-0

EC Name:

Ethyl trans-(±)-2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate hydrochloride

Cas Number:

27107-79-5

IUPAC Name:

27107-79-5

Details on test material:

- Substance name: Trans-Tilidin-HCl-Hemihydrate
- Aggregate state/colour: solid (powder)/white
- Homogeneity: homogeneous
- Storage conditions: ambient, under N2 and light exclusion.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start (t=0h) and the end (t=72h) of the test the uninoculated replicates were sampled.

Test solutions

Details on test solutions:

The test substance was stirred in deionized water for about 10 min at approximately 20±2 °C. The stock solution was clear and colourless. The nominal concentration of the stock solution was 125.3 mg/L. By diluting this stock solution the final test concentrations were prepared.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

The test strain of Desmodesmus subspicatus CHODAT SAG 86.81 is obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and is kept in liquid culture in the Laboratory of Experimental Toxicology and Ecology.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23 ± 2 °C

pH:

7.8-8.3

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L (based on range finding study)
Test concentrations of 0, 6.25, 25 and 100 mg/L were analytically verfified at t=h0 and t=72h.
- Measured concentrations (t=0h): n.d., 6.34, 25.2 and 102.1 mg/L.
- Measured concentrations (t=72h): n.d., 6.25, 23.7 and 92.7 mg/L.

Details on test conditions:

- Test vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Test medium: prepeared according to Guideline.
- Test volume: 100 mL
- Seed-culture: a seed culture was incubated for 4 days at 23±2 °C. Final cell density was 154E+04 cells/mL.
- Pre-culture: the seed-culture was taken to inoculate a pre-culture (initial cell density: 1.0E+04 cells/mL). The pre-culture was incubated for 3 days at 23±2° C. Final cell density was 58E+04 cells/mL.
- lnoculation density (cells/mL): 1.0E+04
- Number of replicates/conc.: 3
- Number of replicates/control: 3
- Illumination: artificial light, type universal white (OSRAM L 25), permanent illumination
- Light intensity: about 60 - 120 µE/(m²·s) at a wave length of 400 - 700 nm
- Test parameter: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) after 0, 24, 48 and 72 h
- Cell counting: after 72 h in a counting chamber (Neubauer improved) in replicate No. 2 of the inoculated control

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

14.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

65.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

72-h EbC50 = 0.48 mg/L (Date of the last control experiment: 25 November 2002).

Any other information on results incl. tables

Calculation of growth rate and inhibition of growth rate after 72 h:

Nominal test concentration (mg/L)	Growth rate (-)	Inhibition (%)
0	0.061	-
1.56	0.058	4.2
3.13	0.060	1.6
6.25	0.059	2.6
12.5	0.056	7.7
25	0.049	19.0
50	0.037	39.7
100	0.021	65.9