Glycerides, C8-18 and C18-unsatd.

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Jan. 25, 2010 to March 5, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study was conducted according to standard guidelines (with GLP)

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Type of method:

column elution method

Test material

Test material information

Constituent 1

Reference substance name:

Glycerides, C8-18 and C18-unsatd.

EC Number:

266-946-3

EC Name:

Glycerides, C8-18 and C18-unsatd.

Cas Number:

67701-28-4

Molecular formula:

Triglycerides containing a glycerol backbone esterified to fatty acids with a carbon chain length of C8-18 as well as unsaturated C18 fatty acids

IUPAC Name:

Tri(C8-18 and C18-unsatd. fatty acyl)glycerol

Details on test material:

- Name of test material (as cited in study report): Fully hydrogenated coconut oil (CAS N° 84836-98-6, EC N° 284-283-8); under the SDA nomenclature, the name of this substance is ‘Glycerides, C8-18, C18 unsaturated’
- Physical state: Solid (bright yellow)
- Expiration date of the lot/batch: December 26, 2009
- Storage condition of test material: In original container, at room temperature (10-30 °C), in the dark

Results and discussion

Water solubility

Water solubility:

< 6.8 mg/L

Temp.:

20 °C

pH:

>= 7 - <= 7.1

Remarks on result:

other: the water solubility was estimated to be below the limit of quantification

Details on results:

- Calibration range: 1 to 20 mg carbon/L pure water in the case of TC-calibration; 1 to 20 mg carbon/L pure water in the case of IC-calibration.
- Linearity of response: Correlation of peak area of different standard solutions with their corresponding concentrations, using a linear regression.
- Regression coefficient (r²): At least 0.999 in the case of TC; at least 0.998 in the case of IC
- Typical calibration curve: y = 4.278* x + 2.2242 in the case of TC; y = 4.232* x – 0.239 in the case of IC
- LOD TC: 0.900 mg carbon/L
- LOD IC: 1.392 mg carbon/L
- LOQ TC: 3.149 mg carbon/L
- LOQ IC: 4.772 mg carbon/L

The limit of quantification TOC (LOQ TOC) was determined as the lowest fortification level at which acceptable mean recovery (70 to 110% of nominal) with a relative standard deviation (RSD) <20% was obtained. The fortified samples were prepared with TC standard solutions in an additional TOC-analysis.

- LOQ TOC: 5 mg carbon/L
- Mean recovery in the fortified samples: 96 % (n = 9, RSD 1 %)

The water solubility of the test item was estimated to be below the limit of quatification of the analytical method.

Any other information on results incl. tables

None

Applicant's summary and conclusion

Conclusions:

The water solubility of fully hydrogenated coconut oil was estimated to be below the limit of quantification of the analytical method and was determined to be < 6.8 mg/L at 20 ± 2 °C.

Executive summary:

The water solubility of fully hydrogenated coconut oil was determined using the coloumn elution method according to OECD Guideline 105 and EU Method A.6 with GLP compliance.

The test flasks, containing carrier material loaded with the test item solution, fully hydrogenated coconut + tetrahydrofuran (which was later removed by rotary evaporator), were stored in darkness at ambient temperature until the test was started. The carrier material was later poured into a coloumn (together with distilled water) which was connected to a pump. In the first experiment the coloumn flow was adjusted to 0.4 mL/min and in the second experiment the flow rate was adjusted to 0.2 mL/min.

The saturation concentration was estimated using the TOC-method.

The water solubility of fully hydrogenated coconut oil was estimated to be below the limit of quantification of the analytical method and was determined to be < 6.8 mg/L at 20 ± 2 °C.