Corn, steep liquor

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment.

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-2 (Acute Dermal Toxicity)

Deviations:

not specified

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Lactic acid

EC Number:

200-018-0

EC Name:

Lactic acid

Cas Number:

50-21-5

Molecular formula:

C3H6O3

IUPAC Name:

2-hydroxypropanoic acid

Details on test material:

- Name of test material (as cited in study report): L(+) lactic acid 80%, diluted with water

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

Approx 24 hours after clipping the skin were abraided sufficiently deep to penetrate the stratum corneum but not the dermis. After test substance application, the trunk of each animal was wrapped.

Duration of exposure:

After a 24 hour exposure period, each binder was removed and the test site of the animal was wiped to remove the remaining test article.

Doses:

2 g/kg

No. of animals per sex per dose:

10

Control animals:

not specified

Details on study design:

Animals were observed for mortality and abnormal clinical signs hourly after dosing on day 0. Observations for mortality and abnormal clinical signs were done twice daily thereafter during the duration of the study (14 days). On day 14 all animals were rendered unconscious and were exsanguinated prior to gross necropsy.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

All animals survived the 14-days duration of the study.

Clinical signs:

other: No abnormal clinical signs were observed during the study. Severe erythema and severe oedema were observed at the test sites of all animals after removal on day 1. Erythema decreased in severity for 3 animals on day 12 or 14, and was not observed for one

Gross pathology:

Multiple depressions in the treated skin were observed during necropsy of one of the same males, of 2 other males , and of one other female. A dark red focus was observed on the lung of one male. No other abnormalities were observed during necropsy of all males and 4 females, and no abnormalities were observed during necropsy of one female.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: CLP (EC 1272/2008)