Chrome tungsten titanium buff rutile

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (missing tester strain to detect crosslinking/oxidising agents, non-GLP)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

missing tester strain to detect crosslinking/oxidising agents

Principles of method if other than guideline:

Standard plate test (repeated with identical concentrations)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Manganese antimony titanium buff rutile

EC Number:

270-185-2

EC Name:

Manganese antimony titanium buff rutile

Cas Number:

68412-38-4

Molecular formula:

(Ti, Sb, Mn) O2

IUPAC Name:

manganese antimony titanium buff rutile

Details on test material:

- Storage condition of test material: 4°C

Method

Target gene:

his-

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

induced rat liver S-9 mix

Test concentrations with justification for top dose:

0, 20, 100, 500, 2500 and 5000 ug/ plate

Vehicle / solvent:

DMSO

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: with S-9 mix: 10 ug 2-aminoanthracene (all strains); without S-9 mix: 5 ug N-methyl-N'-nitro-N-nitroso-guanidine for strains TA100 & TA1535; 10 ug 4-nitro-o-phenylenediamine for strain TA98; 100 ug 9-aminoacridine chloride monohydrate for strain TA 1537

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)


NUMBER OF REPLICATIONS: 3

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Complete solubility of the test substance in DMSO.

Any other information on results incl. tables

1st Standard Plate Test
Solvent: DMSO
Strain	Metabolic activation system	Replicates	maximum revertant factor	dose dependency	Assessment
TA 1535	no	3	1.2	yes	negative
	yes	3	1.3	no	negative
TA 100	no	3	1.1	no	negative
	yes	3	1.1	no	negative
TA 1537	no	3	1.3	no	negative
	yes	3	1.4	no	negative
TA 98	no	3	1.1	no	negative
	yes	3	1.0	no	negative
2nd Standard Plate Test
Solvent: DMSO
Strain	Metabolic activation system	Replicates	maximum revertant factor	dose dependency	Assessment
TA 1535	no	3	1.0	no	negative
	yes	3	1.3	no	negative
TA 100	no	3	1.1	no	negative
	yes	3	1.0	no	negative
TA 1537	no	3	0.9	no	negative
	yes	3	1.1	no	negative
TA 98	no	3	1.1	no	negative
	yes	3	1.1	no	negative