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EC number: 201-058-1 | CAS number: 77-78-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No metabolic activation used, no historical data.
Data source
Reference
- Reference Type:
- publication
- Title:
- Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium
- Author:
- Skopek, T.R.
- Year:
- 1 978
- Bibliographic source:
- Proc. Natl. Acad. Sci. 75, 4465-4469
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- : no metabolic activation used. Not enough test concentrations.
- GLP compliance:
- no
- Remarks:
- Study was conducted before implementation of GLP.
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dimethyl sulphate
- EC Number:
- 201-058-1
- EC Name:
- Dimethyl sulphate
- Cas Number:
- 77-78-1
- Molecular formula:
- C2H6O4S
- IUPAC Name:
- dimethyl sulfate
- Details on test material:
- - Name of test material (as cited in study report): Dimethyl sulphate
- Substance type: technical product
- Physical state: liquid, volatile
- Analytical purity: not stated
- no further significant details stated
Constituent 1
Method
- Target gene:
- not applicable
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 100, 200, 300 uM
- Vehicle / solvent:
- - Phosphate buffered saline
- no further significant details stated
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Remarks:
- with metabolic activation
Migrated to IUCLID6: 40, 60 and 80 µM
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- without metabolic activation
Migrated to IUCLID6: 5, 10 and 15 mM
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- without metabolic activation
Migrated to IUCLID6: 2.5, 5.0 and 7.5 mM
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
DURATION
- Preincubation period: 1 hour
- Exposure duration: 1 hour in a 37°C dry-air incubator without shaking.
NUMBER OF REPLICATIONS: The compounds were delivered to each of duplicate flasks.
EVALUATION: After treatment, the cells were centrifuged, washed and plated on a minimal E agar with biotin and no histidine to determine the his+ revertant fraction. The fraction of revertant colonies in relation to the clones on toxicity plates were calculated.
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth:
An aliquot of cell suspension was added to 0.9 ml of top agar, and the resulting mixture was plated on a minimal E agar plate containing biotin and 5 mM histidine to determine toxicity. - Evaluation criteria:
- no data
- Statistics:
- not madatory for this test system
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- see table 1 under "any other information on results"
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- see table 1 under "any other information on results"
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- No further data
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 Induced mutant fractions of DMS:
Strain |
DMS concentrations |
||
100 µM |
200 µM |
300 µM |
|
TA1535 |
100 |
190 |
420 |
TA1537 |
110 |
220 |
2100 |
TA1538 |
0.6 |
7.8 |
30 |
TA98 |
0 |
20 |
83 |
TA100 |
110 |
340 |
870 |
Table 2: Minimal concentration to which each strain is sensitive
Strain |
Concentration [µM] |
TA 1535 |
4.3 |
TA1537 |
11 |
TA 1538 |
180 |
TA98 |
140 |
TA100 |
32 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive without metabolic activation
DMS induces a substential increase in revertant colony numbers in reverse mutation assay under the described test conditions.
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