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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was conducted in compliance with Japanese Kanpogyo Notification No. 5, Yakuhatsu Notification No. 615 and 49 Kikyoku Notification No. 392. Not all parts of the study are available. All three test concentrations were significantly greater than the measured water solubility of the substance (0.002 mg/L) and test solutions most likely were present as dispersions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Kanpogyo Notification No. 5, Yakuhatsu Notification No. 615, 49 Kikyoku Notification No. 392
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-{[4'-(3-aminophenoxy)-[1,1'-biphenyl]-4-yl]oxy}aniline
EC Number:
700-102-1
Cas Number:
105112-76-3
Molecular formula:
C24H20N2O2
IUPAC Name:
3-{[4'-(3-aminophenoxy)-[1,1'-biphenyl]-4-yl]oxy}aniline
Details on test material:
- Name of test material (as cited in study report): 4,4'-bis(4-aminophenoxy)biphenyl
- Molecular formula (if other than submission substance): C24H2O2N2
- Molecular weight (if other than submission substance): 368.44
- Physical state: White powder
- Analytical purity: 99.98 %
- Lot/batch No.: 89-42001
- Storage condition of test material: Protected from light at room temperature
Radiolabelling:
no

Test solutions

Vehicle:
no
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION OF SPIKED SEDIMENT
- Pooling or mixing of different substrates:
- Method of mixing:
- Details of spiking:
- Equilibration time:
- Equilibration conditions:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution):
- Evaporation of vehicle before use:


PREPARATION OF SPIKED WATER
- Details of spiking: Two grams of test substance were dissolved in 40 mL of dimethylsulfoxide. Two hundred grams of Tween 80 were dissolved in the solution obtained. The solution obtained was then transferred into a 2-L measuring flask with dechlorinated tap water and a dispersed stock solution (concentration of the test substance 1000 mg/L) was obtained after adding water to volume.
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethylsulfoxide, Tween 80
- Concentration of vehicle in final test solution: Group 1 had a concentration of 200 ug/L, Group 2 had a concentration of 20 ug/L.

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
no data

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk

Test conditions

Hardness:
no data
Test temperature:
no data
pH:
no data
Dissolved oxygen:
no data
TOC:
no data
Salinity:
no data
Details on test conditions:
Dechlorinated tap water: 1152 L/day
Nominal and measured concentrations:
Group 1: 200 microgram/L
Group 2: 20 microgram/L
Group 3: 5 microgram/L
Reference substance (positive control):
not specified
Details on estimation of bioconcentration:
The bioconcentration was determined by measuring the the concentration of the test substance in different body tissues of the test fish using HPLC.

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
108 - 254
Basis:
whole body w.w.
Remarks on result:
other: Conc.in environment / dose:200 ug/L
Type:
BCF
Value:
617 - 1 324
Basis:
whole body w.w.
Remarks on result:
other: Conc.in environment / dose:20 ugL
Type:
BCF
Value:
1 596 - 2 590
Basis:
whole body w.w.
Remarks on result:
other: Conc.in environment / dose:5 ug/L
Depuration
Elimination:
yes
Parameter:
DT50
Depuration time (DT):
71 h
Details on kinetic parameters:
no data
Metabolites:
The major metabolites were thought to be acetylated bodies of the test substance.
Results with reference substance (positive control):
no data
Reported statistics:
no data

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

A bioconcentration study was conducted with 3,3'-[biphenyl-4,4'-diylbis(oxy)]dianiline in common carp according to the relevant Japanese guidelines (Mitsui Toatsu Chemicals 1997). Studies over a period of 8 weeks were performed using three concentration levels of 200, 20 and 5 microgram/L. The corresponding bioconcentration factors observed were 108-254, 617-1324 and 1596-2590. Concentration factors corrected for lipid contents in the low dose group were 800-1300, thus comparable to those found in the mid-dose group. An excretion study performed in the high and mid-dose groups revealed half-lives for the retention in the organism of 58.1 and 84.4 hours, respectively.