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EC number: 210-431-8 | CAS number: 615-50-9
Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
Description of key information
The removal / primary biodegradation in an activated sludge unit was determined for the test substance, 2 -methyl-p-phenylenediamine sulfate, over a test period of 98 days according to OECD Guideline 303A.
During acclimitization the activated sludge units were inoculated with activated sludge. The removal of DOC reached a degradation rate >80% after 2 days. After the DOC removal was constant at >80%, the adaptation started with the dosage of the test substance.
During the plateau phase the removal / primary degradation of the test substance was in the range of 84 -95%. The mean removal / primary degradation was 93 +/- 3%, based on n=23 measurements during the 26 day plateau phase.
Key value for chemical safety assessment
Additional information
The removal / primary biodegradation in an activated sludge unit was determined for the test substance, 2 -methyl-p-phenylenediamine sulfate, over a test period of 98 days according to OECD Guideline 303A.
During acclimitization the activated sludge units were inoculated with activated sludge. The removal of DOC reached a degradation rate >80% after 2 days. After the DOC removal was constant at >80%, the adaptation started with the dosage of the test substance.
The nominal concentration of the test substance in the influent was 20.0 mg/L (7.7 mg/L). Specific analysis of the test substance was carried out via HPLC-UV and diode array detector (DAD). Measured concentrations of the test material in the influent and effluent were used to calculate removal.
During the plateau phase the removal / primary degradation of the test substance was in the range of 84 -95%. The mean removal / primary degradation was 93 +/- 3%, based on n=23 measurements during the 26 day plateau phase.
This CAS test was valid according to the OECD 303A guideline.
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