O,O,O-triphenyl phosphorothioate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(10/14 hour dark/light cycle, acclimation period = 4 days)

GLP compliance:

no

Remarks:

prior GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

O,O,O-triphenyl phosphorothioate

EC Number:

209-909-9

EC Name:

O,O,O-triphenyl phosphorothioate

Cas Number:

597-82-0

Molecular formula:

C18H15O3PS

IUPAC Name:

O,O,O-triphenyl thiophosphate

Test animals

Species:

rat

Strain:

other: Tif RAIF (SPF)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ciba Geigy Ltd.
- Weight range at study initiation: 160 - 180 g
- Fasting period before study: overnight
- Housing: 5/cage (macrolon cages type IV)
- Diet: ad libitum; NAFAG Gossau SG
- Water: ad libitum
- Acclimation period: 4 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 10/14

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 50 %
- Justification for choice of vehicle: no data

MAXIMUM DOSE VOLUME APPLIED: no data

DOSAGE PREPARATION: The test substance was suspended with polyethylene glycol (PEG 400). Before treatment the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer.

Doses:

4,640, 7,750 and 10,000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: at least once (before application)
- Frequency of observation: after 1, 24, 48 hours, on day 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Mortality:

One animal died in the mid dose on day 14. No deaths were recorded in the low and high doses.

Clinical signs:

other: Within two hours of dosing, rats in all dose groups experienced sedation, dyspnoea, exophthalmus, curved position and ruffled hair. The surviving animals recovered within 8 days.

Gross pathology:

No substance related gross organ changes were seen.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met