4-(4-Allyloxy-benzenesulfonyl)-phenol

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 April to 07 May 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study undertaken at GLP accredited laboratory to internationally accepted guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

BPS-MAE

IUPAC Name:

BPS-MAE

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): BPS-MAE
- Physical state: White powder
- Analytical purity: 99.2%
- Lot/batch No.: G130131
- Expiration date of the lot/batch:
- Stability under test conditions:
- Storage condition of test material: Desiccator at room temperature.
- Other: Date of receipt: 14 February 2003

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms:On days 5, 7, 14, 21 and 28.
- Sampling intervals/frequency for test medium samples: On days 0, 5, 7, 14, 21 and 28.
- Sample storage conditions before analysis: no data
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
From a acetonitrile (HPLC reagent grade) solution containing 1000mg/L the test substance, 0.2 mL was taken and added to a pair of control fish, which was then analyzed to evaluate recovery. Added to a fishweight of 10 g, the test substance would exist at 20 pg/g, which is 100 times of the high concentration level (0.2 mg/L). The four measurements of recovery gave a mean value of 91.6%, a standard deviation of 0.7% and a range of 1.3%, which is satisfactory for quantitative analysis. The average recovery was used to correct the measured values.

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Identity and concentration of auxiliary solvent for dispersal:
2-methoxyethanol = 25 ppm (v/v) used for high concentration level (0.2 mg//l), low concentration level (0.02 mg/l) and control.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Commercial fish supplier
- Age at study initiation: < 1 year
- Length at study initiation: 8 ± 4 cm
- Weight at study initiation: approx 5 g
- Weight at termination (mean and range, SD):
- Method of breeding: no data
- Health status: good
- Description of housing/holding area: 56l glass aquarium with 50l of water.
- Feeding during test
- Food type: Solution
- Amount: 20 ml
- Frequency: daily

ACCLIMATION
- Acclimation period: >= 2 weeks
- Acclimation conditions: same as test
- Type and amount of food: Babygold from Kyorin; The food was given in an amount of 2% of the fish weight.
- Feeding frequency: daily except week-ends.
- Health during acclimation (any mortality observed): < 5%

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

7 - 28 d

Total depuration duration:

0 d

Test conditions

Hardness:

73 mg/l CaCO3

Test temperature:

Test (solution) temperature: 24 °C (min.) ... 26 °C (max.)

pH:

Test (solution) pH: 6 (min.) ... 8.5 (max.)

Dissolved oxygen:

>= 60% of the saturation

Details on test conditions:

Duration of test: 28 d

Nominal and measured concentrations:

Concentration of test material used: 0.02 amd 0.2 mg/L

Reference substance (positive control):

no

Results and discussion

Lipid content

Lipid content 1

Lipid content:

6.5 - 7.7 %

Time point:

start of exposure

Lipid content 2

Lipid content:

5.7 - 6.8 %

Time point:

end of exposure

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

>= 16 - <= 24 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.02 mg/l

Bioaccumulation factor 2

Type:

BCF

Value:

>= 3 - <= 8 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.2 mg/l

Depuration

Depuration time (DT):

0 min

Details on results:

- Mortality of test organisms: none
- Behavioural abnormalities: none
- Observations on body length and weight: no data
- Organ specific bioaccumulation: no data
- Mortality and/or behavioural abnormalities of control: none
- Loss of test substance during test period:no data

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the BCF obtained, it can be concluded that the possibility for fish to bioaccumulate the test substance is slight under the prescribed test condition.