ethyl 2-(4-phenoxyphenyl)lactate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC Method C2

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

429-220-9

EC Name:

-

Cas Number:

132584-17-9

Molecular formula:

C17 H18 O4

IUPAC Name:

ethyl 2-hydroxy-2-(4-phenoxyphenyl)propanoate

Test material form:

liquid

Specific details on test material used for the study:

Purity: 73.5%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

DMF

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: DMF at a maximum 0.1 ml/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna, < 24 hours old
- Source: Haskell Laboratory, collected from the 9th brood of 27-day old parent daphnids
- Feeding during test: yes
- Food type: Ankistrodesmus falcatus and Selenastrum capricornutum
- Amount: Rate of 125,000 cells/mL of each species (total 250,000 cells/mL
- Frequency: each Monday, Wednesday, and Friday

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

111-114 mg CaCO3/L

Test temperature:

20.4-20.7 degC

pH:

7.4-7.9

Dissolved oxygen:

start: 12.6-13.6 mg/L
48 h: 8.5-8.6 mg/L

Conductivity:

215-238 μmhos/cm

Nominal and measured concentrations:

nominal: 0.046, 0.10, 0.23, 0.51, 1.1, and 2.5 mg/L
measured: 0.032, 0.071, 0.19, 0.38, 0.83, and 2.1 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.38 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.38 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.83 mg/L

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.1 mg/L

Details on results:

%Concentration loss over test: 0 ... 6

Exposure of daphnids to nominal concentrations of IN-JG303 (unadjusted for a.i. content) of 0.046, 0.10, 0.23, 0.51, 1.1, and 2.5 mg/L (corresponding to 0.032, 0.071, 0.19, 0.38, 0.83, and 2.1 mg/L mean, measured IN-JG303 a.i. concentrations, respectively) resulted in 0, 0, 25, 55, 75, and 100% immobility, respectively, at the end of 48 hours. Immobility was not seen in the water and DMF control daphnids.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48-hr EC50 (daphnia magna) = 0.38 mg/L