Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 432-550-6 | CAS number: 243858-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 7, 1999 to Aug. 23, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- -Pretests:
pH 4.0 and 50°C: 2.4 and 120 h
pH 7.0 and 50°C: 2.4 and 120 h
pH 9.0 and 50°C: 2.4 and 120 h
-Main test:
Test 1 at pH 4.0 and 50°C: 6.50, 9.00, 15.30, 20.20 and 24.40 h
Test 1 at pH 7.0 and 50°C: 2.00, 3.00, 3.50, 4.50 and 5.50 h
Test 3 at pH 4.0 and 60°C: 0.17, 4.00, 7.50, 17.70 and 19.40 h
Test 3 at pH 4.0 and 70°C: 0.08, 4.50, 5.00 and 6.00 h
Test 3 at pH 7.0 and 60°C: 0.17, 0.75, 1.50 and 2.50 h
Test 3 at pH 7.0 and 70°C: 0.08, 0.33 and 0.50 h - Buffers:
- - pH: pH 4, 7 and 9
-pH 4:
21.008 g citric acid monohydrat were dissolved in 200 mL sodium hydroxide solution (c=1 mole/L). This solution was filled up to a volume of 1,000 mL with distilled water. 44 mL of hydrochloric acid (c=1 mole/L) were added to 560 mL of this solution and filled up to a volume of 1,000 mL with distilled water. For performing the hydrolysis the solution was refluxed and the pH value was adjusted to pH 4 for the each hydrolysis temperature.
- pH 7:
13.61 g potassium dihydrogen phosphate were dissolved in 1,000 mL distilled water. 29.63 mL of sodium hydroxide solution (c=1 mole/L) were added to 500 mL of this solution and filled up to a volume of 1,000 mL with distilled water. For performing the hydrolysis the solution was refluxed and the pH value was adjusted to pH 7 for each hydrolysis temperature.
- pH 9:
7.46 g potassium chloride and 6.184 g boric acid were dissolved in 1,000 mL water. 500 mL of this solution were added to 21.3 mL sodium hydroxide (c=1 mole/L) and filled up to a volume of 1,000 mL with water. For performing the hydrolysis the solution was refluxed and the pH value was adjusted to pH 9.0 for the each hydrolysis temperature. - Details on test conditions:
- Choice of Concentration:
The optimal concentration of the test substance required for the analysis varied between 136 and 257 mg/L. This value is less than half of the solubility in water and thus satisfies the requirements of the guidelines.
Preparation of Test Samples:
Solutions of the test substance were prepared by weighing between 7 and 13 mg of the test substance in a 50 mL volumetric flask and dissolving in the relevant buffer. The resulting solution was subjected immediately to the hydrolysis procedure.
Preparation of the Standards:
The calculation standards were freshly prepared by weighing between 7 and 13 mg of the standard test substance in a 50 mL volumetric flask and dissolving in the buffer pH 4 or pH 7.
Hydrolysis Test Procedure:
The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at the selected temperature for the specified time. Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay. - Positive controls:
- no
- Preliminary study:
- The decomposition at pH 9 was higher than 50% after 2.4 h at 50°C. Thus the test substance was considered hydrolytically instable at pH 9. According to the guideline a half-life period of less than 1 d under environmental conditions (25°C) was estimated. The pretests at pH 4 and at pH 7 indicated that an examination of the hydrolysis kinetics is essential.
- Transformation products:
- not specified
- % Recovery:
- >= 93.4 - <= 94
- St. dev.:
- 0.3
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- >= 7.1 - <= 7.7
- St. dev.:
- 0.3
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- >= 65.4 - <= 66.3
- St. dev.:
- 0.4
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- >= 0.7 - <= 1.3
- St. dev.:
- 0.3
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- >= 0.45 - <= 0.46
- St. dev.:
- 0.1
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- >= 0.06 - <= 0.36
- St. dev.:
- 0.2
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.025 h-1
- DT50:
- 27.63 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 60 °C
- Hydrolysis rate constant:
- 0.056 h-1
- DT50:
- 12.45 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 70 °C
- Hydrolysis rate constant:
- 0.133 h-1
- DT50:
- 5.21 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.186 h-1
- DT50:
- 3.73 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 60 °C
- Hydrolysis rate constant:
- 0.637 h-1
- DT50:
- 1.08 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 70 °C
- Hydrolysis rate constant:
- 1.634 h-1
- DT50:
- 0.42 h
- Type:
- (pseudo-)first order (= half-life)
- Key result
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- 12.9 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: extrapolated according to the Arrhenius equation
- Key result
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- 3.4 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: extrapolated according to the Arrhenius equation
- Key result
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- < 1 d
- Remarks on result:
- other: based on preliminary study
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the extrapolated half-life for hydrolysis of the test substance was found to be 12.9 and 3.4 d at pH 4 and at pH 7, respectively.
- Executive summary:
A study was conducted to determine hydrolysis as a function of pH for the test substance according EU Method C.7 and in compliance with GLP.
Based on the results of a preliminary test which demonstrated test substance decomposition to be higher than 50% after 2.4 h at pH 9 and 50°C, the test substance was considered hydrolytically instable at pH 9. According to the guideline a half-life period of less than 1 d under environmental conditions (25°C) was estimated at pH 9. The pretests at pH 4 and at pH 7 indicated that examination of the hydrolysis kinetics was essential. Hence a main study was conducted at pH 4 and 7.
At pH 4 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70°C to a temperature of 25°C. It was found to be 12.9 d. At pH 7 the extrapolated half-life at the temperature of 25°C was found to be 3.4 d.
Under the test conditions, the extrapolated half life for hydrolysis of the test substance was found to be 12.9 and 3.4 d at pH 4 and at pH 7 respectively.
Reference
Table 1: Summary and extrapolation of the hydrolysis results at pH 4
Temperature | Half life | Experiment |
50°C | 27.63 h | test 1 |
60°C | 12.45 h | test 3 |
70°C | 5.21 h | test 3 |
25°C | 310.4 h = 12.9 d | extrapolation |
Table 2: Summary and extrapolation of the hydrolysis results at pH 7
Temperature | Half life | Experiment |
50°C | 3.73 h | test 1 |
60°C | 1.08 h | test 3 |
70°C | 0.42 h | test 3 |
25°C | 82.1 h = 3.4 d | extrapolation |
Description of key information
Under the test conditions, at pH 4 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70°C to a temperature of 25°C. It was found to be 12.9 d. At pH 7 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70°C to a temperature of 25°C. It was found to be 3.4 d.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 3.4 d
- at the temperature of:
- 25 °C
Additional information
A study was conducted to determine hydrolysis as a function of pH for the test substance according EU Method C.7 and in compliance with GLP. Based on the results of a preliminary test which demonstrated test substance decomposition to be higher than 50% after 2.4 h at pH 9 and 50°C, the test substance was considered hydrolytically instable at pH 9. According to the guideline a half-life period of less than 1 d under environmental conditions (25°C) was estimated at pH 9. The pretests at pH 4 and at pH 7 indicated that examination of the hydrolysis kinetics was essential. Hence a main study was conducted at pH 4 and 7. At pH 4 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70°C to a temperature of 25°C. It was found to be 12.9 d. At pH 7 the extrapolated half-life at the temperature of 25°C was found to be 3.4 d. Under the test conditions, the extrapolated half life for hydrolysis of the test substance was found to be 12.9 and 3.4 d at pH 4 and at pH 7 respectively.
(Muhlberger, 1999).Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.