Reaction mass of Cobaltate(3-), bis[2-[[[3-[2-[1-[[(2-chlorophenyl)amino]carbonyl]-2-(oxo-κO)propyl]diazenyl-κN1]-4-(hydroxy-κO)phenyl]sulfonyl]amino]benzoato(3-)]-, sodium (1:3) and tetrasodium bis[2-[[[3-[[1-[(2-chloroanilino)carbonyl]-2-oxopropyl]azo]-4-hydroxyphenyl]sulphonyl]amino]benzoato(3-)]cobaltate(4-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Section 2

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT - 92'368/A
EN/Batch: PLN 13
Purity: ca. 80 %
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 03/98

Analytical monitoring:

yes

Details on sampling:

Samples for analytical determinations were taken at 0 and 48 h after mixing of the test solutions from the approximate center of the test vessels. The samples were stored at -18 to - 25 °C until analyses.

Vehicle:

yes

Details on test solutions:

Stock solution:
0.5 g test substance was mixed with 200 mL test medium and the volume was then made up to 500 mL with test medium. The stock solution was homogenized for five minutes in an ultrasonic bath.

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia were bred in the testing facility of the Product Ecology TF, Textile Dyes Division under standardised conditions.

BREEDING:
Cultures of Daphnia magna were maintained in glass vessels containing approx. 3.0 L of reconstituted water at 18-22 °C. The water was renewed partially three times weekly. The Daphnia were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food was consumed within 24 h. The quality of the Daphnia was checked every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8-1.5 mg/L).

PRETREATMENT:
24 h before the start of the exposure, reproductive daphnids were separated from the young Daphnia by sieving all individuals through a 710 µm sieve.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

none

Post exposure observation period:

no data

Hardness:

136 mg/L CaCO3 (theoretical water hardness)

Test temperature:

21.6 - 21.8 °C

pH:

7.8 - 8.0

Dissolved oxygen:

The medium was oxygen saturated at the beginning of the test. The oxygen concentrations ranged from 96 - 99 % through the study duration.

Salinity:

no data

Nominal and measured concentrations:

Nominal: 10, 18, 32, 58, 100 and 180 mg/L (test concentrations chosen on the basis of a pre-test).
Measured: 9.2, 17, 32, 55, 98, 175 mg/L (at 0 h) and 9.1, 18, 32, 57, 100 and 175 mg/L (at 48 h).
Average of measured concentrations: 9.2, 18, 32, 56, 99 and 175 mg/L.

During the test duration, the test article concentrations in the analysed test media were in the range of 91.0-100.0% of the nominal values. In all test concentrations non alteration of the test substance after 48 h was observed. Therefore, under the test conditions the test article was sufficiently stable.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL beakers containing 100-200 mL test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Test medium composition: NaHCO3 = 65 mg/L; MgSO4 * 7H2O = 123.3 mg/L; KCl = 5.8 mg/L; CaCI2 * 2H2O = 294 mg/L; Na2SiO3 * 9H2O = 10 mg/L; NaNO3 = 274 µg/L; KH2PO4 = 143 µg/L; K2HPO4 = 184 µg/L
- Dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4: 1
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Photoperiod: 16 h illumination.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 9.2 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 47.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: The EC50 value was calculated with the " Probit model " method. 95 % confidence limit (24h): 38.73 - 57.96

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

175 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 9.2 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

30.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: The EC50 value was calculated with the " Probit model " method. 95 % confidence limit (48h): 26.21 - 35.87

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

56 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 9.2 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations.

Results with reference substance (positive control):

Potassium dichromate 24 h EC50 = 0.97 mg/L

Reported statistics and error estimates:

no data

Table 1: Immobilization of Daphnia

Average measured concentration of test substance (mg/L)	No. of Animals	% immobility
		24 h	48 h
Blank	20	0	0
9.2	20	0	0
18	20	10	10
32	20	10	45
56	20	80	100
99	20	80	100
175	20	100	-

 

Validity criteria fulfilled:

yes

Conclusions:

The test substance is slightly toxic to daphnids with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h, respectively.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance FAT 92368/A to Daphnia magna in a 48 h static test according to the OECD Guideline 202, Section 2, and the EU Method C.2, in GLP-compliance. Daphnia were exposed to nominal test substance concentrations of 10, 18, 32, 58, 100 and 180 mg/L. Analytical determination of the test substance concentration was carried out at 0 and 48 h by spectrophotometry. During the test, the test substance concentrations in the analysed test media were in the range of 91.0 - 100.0 % of the nominal values. No alteration of the test substance was observed after 48 h. The average measured test substance concentrations were 9.2, 18, 32, 56, 99 and 175 mg/L. No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations. Based on the above observations, the 24 and 48 h EC₀ values for the test substance were established at ≥9.2 mg/L, and the 24 and 48 h EC₁₀₀ values were established at 56 and 175 mg/L respectively. The EC50 values were calculated with the ‘Probit model’ method and found to be 47.4 mg/L at 24 h and 30.5 mg/L at 48 h. Hence,based on the above findings, the test substance is considered to be slightly toxic to Daphnia with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h exposures, respectively.

Description of key information

A study was conducted to determine the acute toxicity of the test substance FAT 92368/A toDaphnia magnain a 48 h static test according to the OECD Guideline 202, Section 2, and the EU Method C.2, in GLP-compliance. Daphnia were exposed to nominal test substance concentrations of 10, 18, 32, 58, 100 and 180 mg/L. Analytical determination of the test substance concentration was carried out at 0 and 48 h by spectrophotometry. During the test, the test substance concentrations in the analysed test media were in the range of 91.0 - 100.0 % of the nominal values. No alteration of the test substance was observed after 48 h. The average measured test substance concentrations were 9.2, 18, 32, 56, 99 and 175 mg/L. No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations. Based on the above observations, the 24 and 48 h EC₀values for the test substance were established at ≥ 9.2 mg/L, and the 24 and 48 h EC₁₀₀values were established at 56 and 175 mg/L respectively. The EC50 values were calculated with the ‘Probit model’ method and found to be 47.4 mg/L at 24 h and 30.5 mg/L at 48 h. Hence, based on the above findings, the test substance is considered to be slightly toxic to Daphnia with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h exposures, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

30.5 mg/L